Description

SHENTEK™ Residual HEK293 DNA Size Analysis Kit is used to quantitate HEK293 DNA residues of different fragment sizes for various stages of biopharmaceutical products, from in-process samples to final products. This kit utilizes real-time PCR technology to perform rapid and specific quantitation of residual HEK293 DNA fragments in samples. It is designed to amplify four different fragments (75bp, 122bp, 244bp, 562bp) for the accurate determination of their size distribution with the LOD level of femtograms. For extraction information, please refer to the SHENTEK™ Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).

Introduction

Components	Shipping Condition	Unit Size	Product Line	Detection Method
qPCR Reaction Buffer	-20℃, protect from light	4x100 Reactions	SHENTEK™	Probe-based qPCR
HEK293 Primer&Probe MIX-75
HEK293 Primer&Probe MIX-122
HEK293 Primer&Probe MIX-244
HEK293 Primer&Probe MIX-562
IPC MIX
DNA Dilution Buffer (DDB)	-20℃
Vero DNA Control	-20℃

Specification

Range	3x10-2-3x102 pg/μL, R2 ≥0.990, AE=96.8%-102.1%
Accuracy	Recovery = 76.0%-121.1%, CV<40%
LOQ	0.03 pg/μL
Precision	4.0%-35.4% (<40%)
Robustness	Freeze-thaw stability over 5 cycles
Robustness	Instrument suitability not limited to Thermo, Biorad, Roche, HZSKBio qPCR equipments

Documents

User Guide

Related Products

SHENTEK® Residual Host Cell DNA Sample Preparation Kit

Residual HEK293 DNA Size Analysis Kit SHENTEK™