Introduction

Components	Shipping Condition	Unit Size	Product Line	Detection Method
E.coli RNA Control	-20℃	100 Reactions	SHENTEK™	Probe-based qPCR
One Step qPCR Buffer	-20℃, protect from light
One Step Enzyme MIX	-20℃, protect from light
E.coli RNA Primer&Probe MIX (Incl IPC)	-20℃, protect from light
RNase-Free H2O	-20℃

Overview

SHENTEK™ Residual E. coli RNA Quantitation Kit (2G) is used for quantitation of host cell RNA from E. coli in a variety of biopharmaceutical products. This kit employs duplex reverse transcription quantitative PCR technique to performs a rapid, specific, and reliable quantitative detection of residual E. coli RNA .The target gene (FAM) can be determined at the femtogram (fg) level, whereas IPC-Internal Positive Control (VIC) is also included in the E. coli RNA Primer&Probe MIX to evaluate the performance of each PCR reaction.

Specification

Range	2x10-2-2x102pg/μL, R2 ≥0.990，E=83.3%~110%
Accuracy	64.1%-97.8%, CV<30%
LOQ	0.002 pg/μL
Precision	10.7%-13.1% (<30%)
Robustness	Freeze-thaw stability over 5 cycles
Robustness	Instrument suitability not limited to Thermo, Biorad, HZSKBio qPCR equipments

Related Products

SHENTEK™ Residual Host Cell RNA Sample Preparation Kit

Documents

User Guide

Residual E.coli RNA Quantitation Kit (2G)-SHENTEK™