Background

Recombinant human vitronectin protein efficiently binds to extracellular matrices and is primarily used to promote cell adhesion by coating plates or flasks. In the matrix, vitronectin can support cell adhesion by binding to various integrins and other proteoglycans. In addition, vitronectin can function as a chemically defined matrix component in human embryonic stem cell regeneration media.

Specifications

Protein sequence	A DNA sequence encoding the human Vitronectin (NP_000629.3) was expressed with a polyhistidine tag at the C-terminus
Expression Host	HEK293 Cells
QC Testing Purity	> 90 % as determined by SDS-PAGE
Activity	Vitronectin promotes attachment of hESCs and iPSCs with 5 μg/mL under the condition of serum-free feeding
Endotoxin Level	< 0.1 EU per μg of the protein as determined by the LAL method
Molecular Mass	The Recombinant Human Vitronectin consists of 465 amino acids and predicts a molecular mass of 53 kD
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 6% mannitol are added as protectants before lyophilization
Stability & Storage	Samples are stable for up to 24 months from date of receipt at 4 ℃. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles
Usage method	1.Reconstitute each 20 mg of vitronectin with 1 mL of sterilized water, mix well and stand for 25~30 minutes until dissolving. Then mix well, aliquot and freeze. 2.Dilute vitronectin with sterile phosphate buffer (PBS) to obtain the desired concentration and dissolve vitronectin by gentle pipetting. Note: Do not vortex vigorously; Different concentrations of dilutions need to be tested to determine the optimal concentration for each culture system. Recommended coating concentration range is 5~50 μg/mL. 3.Taking 6-well-plate as an example, add 1 mL of diluted vitronectin solution to each well, and leave the plate at room temperature for 1~2 hours. 4. After the plate is completely coated, aspirate PBS and discard it, and gently wash the plate with sterile PBS.

Data Figures

References

Evaluation of potential effects of Plastin 3 overexpression and low-dose SMN-antisense oligonucleotides on putative biomarkers in spinal muscular atrophy mice.Strathmann EA, Peters M, Hosseinibarkooie S, Rigo FW, Bennett CF, Zaworski PG, Chen KS, Nothnagel M, Wirth B.PLoS One. 2018 Sep 6;13(9):e0203398. doi: 10.1371/journal.pone.0203398. eCollection 2018.
Detection of Complement Activators in Immune Attack Eyes After iPS-Derived Retinal Pigment Epithelial Cell Transplantation.Sugita S, Makabe K, Fujii S, Takahashi M.Invest Ophthalmol Vis Sci. 2018 Aug 1;59(10):4198-4209. doi: 10.1167/iovs.18-24769.
Improving single-cell cloning workflow for gene editing in human pluripotent stem cells.Chen YH, Pruett-Miller SM.Stem Cell Res. 2018 Aug;31:186-192. doi: 10.1016/j.scr.2018.08.003. Epub 2018 Aug .
Bacterial Outer Membrane Vesicles Induce Vitronectin Release Into the Bronchoalveolar Space Conferring Protection From Complement-Mediated Killing.Paulsson M, Che KF, Ahl J, Tham J, Sandblad L, Smith ME, Qvarfordt I, Su YC, Lindén A, Riesbeck K.Front Microbiol. 2018 Jul 13;9:1559. doi: 10.3389/fmicb.2018.01559. eCollection 2018.

Document

GMP-TL651

GMP-TL651_SDS

Recombinant Human Vitronectin Protein