Description

SCF (Stem Cell Factor), also known as KIT ligand or mast cell growth factor, is a glycoprotein that exists in both soluble and membrane-bound forms. It functions by binding to the c-KIT receptor on the cell surface and activating downstream signaling pathways. When used in combination with other cytokines—such as IL-3, IL-6, and FLT3 ligand—SCF promotes the expansion of hematopoietic stem and progenitor cells in vitro. It acts synergistically with EPO to support erythroid progenitor differentiation and with TPO to enhance megakaryopoiesis. In organoid culture systems, SCF helps maintain the activity of intestinal stem cells, mammary stem cells, and other tissue-specific stem cells. The core functions of SCF in cell culture include sustaining stem cell activity, promoting proliferation, and inhibiting apoptosis. It is particularly indispensable in in vitro studies of the hematopoietic system and specific lineage development. Its applications span foundational research, regenerative medicine, and drug development.

Specifications

Product Details
Cat. No.	Y03801
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is 5-8 ng/mL.
Accession	P21583
Alternative Names	Kit Ligand; Mast Cell Growth Factor; MG/VAIL F; Stem Cell Factor; SCF; c-Kit ligand; KITLG; MG/VAIL F; SCF
Species	Human
Expression System	CHO
Mol. Weight	18.5 kDa
Expression Region	Glu 26-Ala 190
Tag Info	Tag free
Formulation	Lyophilized
Buffer	PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Key hematopoietic growth factor, supports HSC proliferation and survival.

Stem Cell Research

Essential for hematopoietic stem cell maintenance and expansion in vitro.

Cell Biology

Regulates stem/progenitor cell survival, proliferation, and adhesion via c-Kit signaling.

Data Figures

Figure 1: Recombinant Human SCF (Cat. No. Y03801) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y03801 SDS-PAGE.

Recombinant Human SCF (Cat. No. Y03801) stimulates proliferation of TF-1 cells. The ED50 for this effect is 5-8 ng/mL. The bioactivity of Y03801 was higher than other competing products.

Figure 2: Recombinant Human SCF (Cat. No. Y03801) stimulates proliferation of TF-1 cells. The ED50 for this effect is 5-8 ng/mL. The bioactivity of Y03801 was higher than other competing products.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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