Description

Stem Cell Factor, also known as SCF, kit-ligand, KL, steel factor, KITLG, FPH2, KL-1, Kitl, MGF, SCF, SF, or SHEP7, is a dimeric molecule that exerts its biological functions by binding to and activating the receptor tyrosine kinase c-Kit. Activation of c-Kit leads to its autophosphorylation and initiation of signal transduction. Signaling proteins are recruited to activated c-Kit by certain interaction domains that specifically bind to phosphorylated tyrosine residues in the intracellular region of c-Kit. Binding of SCF to C-kit induces receptor dimerization and autophosphorylation of tyrosine residues in the cytoplasmic domain. Tyrosine phosphorylation initiates multiple signaling pathways including RAS, PI3 kinase, Src, and JAK/STAT. However, SCF is a versatile factor in the differentiation of many specific cell types like spermatogonial stem cells and megakaryocyte progenitors. Apart from differentiation, SCF also can maintain stemness in cells. For clinical application, SCF is used in combination with other cytokines to generate myeloid-derived suppressor cells from human umbilical cord blood. SCF is also used to generate T cells for cell-based therapies, drug screening and disease modeling. In regenerative studies, SCF is applied in wound healing hydrogel as a means of increasing its adhesion strength and tissue regeneration.

Data Figures

Specifications

Cytokines
Expression Host	HEK293 cells
Synonym	C-kitligand,DCUA,FPH2,FPHH,Kitl,KL-1,MGF,SCF,SF,SHEP7
Protein Sequence	A DNA sequence encoding the human SCF (NP_000890.1) was expressed with a His-tag at the C-terminus.
Molecular Mass	The recombinant human SCF protein consists of 170 amino acids and predicts a molecular mass of 19.3 kD.
QC Testing Purity	> 90 % as determined by SDS-PAGE.
Endotoxin	< 0.1EU per 1 μg of the protein by the LAL method.
Activity	Measured in a cell proliferation assay using Mo7e cells, corresponding to a activity of ≥2.0x10⁵IU/mg.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.
Stability	Lyophilized preparation can be stored at -20 ℃. 6 months at -20℃ under sterile conditions after reconstitution. 12 months at -80℃ under sterile conditions after reconstitution.
Storage	Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL. Avoid repeated freeze-thaw cycles.

Documents

Recombinant Human SCF Protein-GMP

Recombinant Human SCF Protein-RUO

GMP-TL504_SDS.pdf