Description

The recombinant human PDGF-BB-His fusion protein is a high-purity homodimeric growth factor produced in HEK-293 expression systems, featuring a C-terminal polyhistidine tag for nickel-affinity chromatography purification.

As a core member of the PDGF/VEGF superfamily, PDGF-BB specifically engages PDGFR-β to activate Ras-MAPK and PI3K-Akt signaling pathways, driving directed migration, proliferation, and differentiation of mesenchymal stem cells, pericytes, and vascular smooth muscle cells while enhancing extracellular matrix adhesion through integrin αvβ3 upregulation.

In cell therapy applications, this protein serves as a critical component in 3D bioscaffold culture systems, where controlled release kinetics enhance ex vivo expansion and paracrine activation of mesenchymal stem cells (MSCs), synergize with VEGF to engineer functional vascular networks, and promote neural progenitor differentiation into oligodendrocyte precursor cells (OPCs), thereby creating vascularized microenvironments for tissue-engineered grafts.

The native disulfide-bonded dimeric configuration preserves full bioactivity, while the His-tag design enables precise purification without Fc-mediated off-target activation, making it particularly suitable for regenerative medicine applications requiring spatiotemporal control of growth factor concentrations.

Specifications

Product Details
Synonyms	Platelet-derived growth factor subunit B, PDGF-2, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis
Accession	UniProt P01127
Expressed Region	Ser82–Thr190
Tag	C-terminal 6×His-tag
Expression System	HEK293 cells
Predicted Molecular Weight	13.1 kDa
Purity	> 90% as determined by SDS-PAGE
Endotoxin	< 0.1 EU per 1 μg of protein (LAL method)
Formulation	Lyophilized from sterile 20 mM phosphate-buffered saline (PBS), pH 7.4, typically containing 6–8% (w/v) mannitol as a stabilizing protectant

Features

✔ Native Dimeric PDGF-BB Activity
Preserves disulfide-bonded homodimer structure for authentic PDGFR-β signaling.
✔ Potent MSC-Targeted Signaling
Activates Ras-MAPK and PI3K-Akt pathways to drive migration, proliferation, and differentiation of mesenchymal cells.
✔ High Purity, Low Endotoxin
HEK293 expression and His-tag purification ensure consistent quality for sensitive cell culture systems.
✔ Excellent Batch Stability
Reliable performance across batches supports reproducible tissue engineering workflows.

Applications

Mesenchymal Stem Cell (MSC) Expansion

Enhances MSC proliferation and paracrine activity in 2D and 3D culture systems.

Vascularized Tissue Engineering

Synergizes with VEGF to support pericyte recruitment and functional vascular network formation.

3D Bioscaffold-Based Cell Culture

Enables controlled growth factor delivery for spatially organized tissue constructs.

Neural & Regenerative Medicine Research

Supports neural progenitor differentiation and regeneration-oriented microenvironment design.

Stability & Storage

√ Lyophilized powder: Stable for 12 months at -80°C or 6 months at -20°C when stored inthe original sealed container under desiccant.
√ Reconstitution: Dissolve in sterile Water for Injection, 0.9% NaCl, or PBS (pH 7.4)maintaining final concentration ≥100 µg/mL to prevent adsorption.
√ Handling: Aliquot to avoid repeated freeze-thaw cycles.

Cautions

• Research Use Only: This product is not intended for diagnostic or therapeutic use.

• Handling: Follow recommended storage and handling instructions to maintain product stability.

• Support: Contact technical support if assistance is required.

Document

📄 GMP-TL644 Data Sheet 📄 GMP-TL644 SDS 📄 GMP-TL644-0050 COA 📄 GMP-TL644-0100 COA

Recombinant Human PDGF-BB Protein