Description

PDGF-AA (Platelet-Derived Growth Factor-AA), a subtype of the platelet-derived growth factor (PDGF) family, consists of two identical A chains. It primarily regulates cellular processes such as proliferation, migration, differentiation, and survival by activating tyrosine kinase receptors on the cell membrane (PDGFR-αα, PDGFR-αβ, or PDGFR-ββ). PDGF-AA specifically binds to the PDGFR-αα receptor and stimulates the proliferation of specific cell types, including fibroblasts, glial cells, and mesenchymal stem cells (MSCs), making it widely used for expanding primary or hard-to-culture cells. In wound healing and tissue engineering research, PDGF-AA can induce directed cell migration to mimic in vivo repair processes. For certain mesenchymal stem cells or neural precursor cells, it helps maintain their undifferentiated state or promotes directed differentiation—such as toward osteogenic or glial lineages.

Specifications

Product Details
Cat. No.	Y07001H
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	The bioactivity is determined by the dose-dependent proliferation of NIH3T3 cells is typically between ≤5.0 ng/mL.
Accession	P04085
Alternative Names	Platelet-derived growth factor subunit A; PDGF-1
Species	Human
Expression System	CHO
Target Protein Sequence	Ser87-Thr211
Mol. Weight	14.3kDa
Tag Info	Tag free
Formulation	Lyophilized
Buffer	PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Stem Cell Research

Supports mesenchymal stem cell proliferation and migration.

Cell Biology

Stimulates fibroblast proliferation, migration, and tissue remodeling.

Data Figures

Figure 1: Measured in a cell proliferation assay using NIH3T3 cells.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA

Recombinant Human PDGF-AA