Description

Oncostatin M (OSM), a multifunctional cytokine belonging to the interleukin-6 (IL-6) family, is primarily secreted by activated immune cells such as T cells, monocytes, and macrophages. It plays diverse and important roles in cell culture systems and has garnered significant attention in stem cell research, tissue engineering, and disease modeling. Under specific conditions, OSM can inhibit the differentiation of embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs), thereby helping to maintain their pluripotency. Conversely, OSM can also promote differentiation in certain contexts. For example, when used in combination with hepatocyte growth factor (HGF), it induces the differentiation of hepatic progenitor cells into functional hepatocytes. Additionally, OSM stimulates the osteogenic differentiation of mesenchymal stem cells while suppressing adipogenesis.

Specifications

Product Details
Cat. No.	Y05101
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is ≤ 2 ng/mL.
Accession	P13725
Alternative Names	Oncostatin-M; OSM
Species	Human
Expression System	CHO
Mol. Weight	22 kDa
Expression Region	Ala26-Arg221
Tag Info	Tag free
Formulation	Lyophilized
Buffer	PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Supports megakaryocyte proliferation and differentiation.

Immunology

Modulates inflammatory responses and immune cell activation.

Stem Cell Research

Promotes differentiation of hematopoietic progenitors and certain stem cell lineages.

Cell Biology

Regulates cell proliferation, differentiation, and tissue remodeling via JAK/STAT pathways.

Data Figures

Figure 1: Recombinant Human OSM (Cat. No. Y05101) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y05101 SDS-PAGE.

‍Human OSM (Cat. No. Y05101) stimulates proliferation of TF-1 cells. The specific bioactivity of Recombinant Human OSM is ≤ 2 ng/mL. The bioactivity of Recombinant Human OSM (Cat. No. Y05101) was higher than the other competing product.

Figure 2: ‍Human OSM (Cat. No. Y05101) stimulates proliferation of TF-1 cells. The specific bioactivity of Recombinant Human OSM is ≤ 2 ng/mL. The bioactivity of Recombinant Human OSM (Cat. No. Y05101) was higher than the other competing product.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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