Description

Recombinant Human Neuregulin-1 beta 1 (NRG1-β1) is a member of the neuregulin family of growth factors that plays a critical role in cell proliferation, differentiation, and survival. It functions by binding to ErbB receptor tyrosine kinases (ErbB3 and ErbB4), activating downstream signaling pathways such as PI3K/AKT and MAPK. NRG1-β1 is widely involved in cancer biology, neural development, and cell communication.

This recombinant protein is expressed in E. coli and corresponds to the functional region (S177–E241) of human NRG1-β1, retaining its biological activity. With high purity (≥95%) and validated bioactivity (ED50: 1–8 ng/mL in MCF-7 cell proliferation assay), this product is suitable for a wide range of applications in oncology and cell signaling research. The protein is supplied in a tag-free, lyophilized form for convenient storage and reconstitution.

Specifications

Product Details
Cat. No.	Y01621H
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a cell proliferation assay using MCF-7 breast cancer cell line.The ED50 for this effect is 1-8 ng/mL.
Accession	NP_039250
Alternative Names	/
Species	Human
Expression System	E. coli
Mol. Weight	7.5 kDa
Expression Region	S177-E241
Tag Info	Tag free
Formulation	Lyophilized
Buffer	Lyophilized from a 0.22 μm-filtered solution containing 10 mM PB, 5% Trehalose and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock,but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of ≥95%, ensuring reliable and reproducible experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Optimized Expression System: Expressed in E. coli, enabling efficient production and suitability for routine laboratory applications.
✔ Convenient and Reliable: Supplied in a tag-free, lyophilized form for easy reconstitution, stable storage, and compatibility with various experimental workflows.

Applications

Neuroscience

Involved in neural development and cell communication, supporting research on nervous system function and disorders.

Cancer Research

Widely used in studies of tumor progression and signaling, especially in breast cancer models such as MCF-7 cells.

Cell Biology

Regulates cell proliferation, differentiation, and survival via ErbB receptor-mediated signaling pathways.

Data Figures

Measured in a cell inhibitionn assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.2 ng/mL. The bioactivity of Y07901 was higher than other competing products.

Figure 1: Measured in a cell inhibitionn assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.2 ng/mL. The bioactivity of Y07901 was higher than other competing products.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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Recombinant Human NRG1-β1