Description

Noggin is a secreted dimeric glycoprotein that is widely expressed in vertebrates and is highly conserved among different vertebrate species.

Noggin is a key regulator that modulates the formation of ectoderm-, mesoderm-, and endoderm-derived tissues and plays a critical role in bone development, joint formation, and neural tube closure.

Noggin regulates the BMP signaling pathway by binding to BMPs and preventing them from interacting with receptors.

As an antagonist of BMPs, Noggin inhibits chondrocyte proliferation and thus ensures proper joint formation.

When culturing human embryonic stem cells (hESCs) or neural stem cells under certain conditions, adding Noggin to antagonize BMP activity enables stem cells to proliferate while maintaining their undifferentiated state.

Specifications

Parameter	Specification
Expression Host	CHO cells
Synonyms	Noggin; NOG; SYM1; SYNS1
Protein Sequence	Amino acid sequence: Gln28–Cys232 of human Noggin (UniProt ID: Q13253)
Molecular Weight	Predicted molecular weight is 23.05 kDa
Purity	>95%, analyzed by SDS-PAGE
Endotoxin	<0.05 EU/μg
Purification Method	Chromatographic purification
Appearance	White lyophilized powder
Activity	Refer to COA for details

Features

✔ CHO Cell–Expressed Human Noggin (Gln28–Cys232)
Recombinant human Noggin corresponding to Gln28–Cys232 produced in CHO cells with a predicted molecular weight of 23.05 kDa.
✔ High Purity Chromatographic Preparation
Purity >95% confirmed by SDS-PAGE analysis following chromatographic purification.
✔ Low Endotoxin Profile
Endotoxin level <0.05 EU/μg supporting sensitive in vitro cell culture applications.
✔ Defined Lyophilized Protein Format
Supplied as a white lyophilized powder with verified electrophoretic identity and consistent protein quality.

Application

BMP Signaling Antagonism Studies

Binds BMP family proteins and prevents receptor interaction, enabling investigation of BMP signaling pathways.

Stem Cell Culture Systems

Used in stem cell culture models including MSC and iPSC systems to modulate BMP signaling during cell expansion.

Embryonic Development Research

Applied in studies involving ectoderm-, mesoderm-, and endoderm-derived tissue formation and developmental biology.

Joint and Skeletal Development Models

Supports research on bone development, joint formation, and chondrocyte proliferation regulation.

Storage

Store below -20°C

Instructions

Centrifuge before reopening the tube. Dissolve the lyophilized protein in distilled water. It is recommended to reconstitute to a concentration greater than 100 μg /mL.

After reconstitution, the protein can be stored at -80℃ for 3-6 months and at 2-8℃ for 2-7 days. For optimal storage, aliquot the protein and minimize freeze-thaw cycles.

Precautions

This product is only suitable for in vitro cell culture and cannot be used directly in clinical treatment.

Documents

📄 TL-642 Data Sheet 📄 TL-642 SDS 📄 TL-642 COA

Recombinant Human Noggin Protein