Instructions

Centrifuge before reopening the tube. Dissolve the lyophilized protein in distilled water. It is recommended to reconstitute the concentration to greater than 100 μg /mL.

After reconstitution, the protein can be stored at -80°C for 3-6 months and at 2-8°C for 2-7 days. For optimal storage, aliquot the protein and minimize freeze-thaw cycles.

Specifications

Expression host	CHO cells
Synonyms	Noggin , NOG , SYM1 , SYNS1
Protein sequence	DNA sequence encoding human Noggin ( Q13253 ) expressed in CHO cells
Molecular weight	Predicted molecular weight is 23.05kD
Purity	> 95%, analyzed by SDS-PAGE
Endotoxin	<0.05EU/ μg
Purification method	Purification method
Appearance	White loose body

Applications

Noggin is a secreted dimeric glycoprotein that is widely expressed in vertebrates and has high homology among different vertebrate species . Noggin is the main metabolite that regulates the formation of ectoderm-, mesoderm-, and endoderm-derived tissues, and plays a key role in bone development, joint formation, and neural tube fusion. Noggin regulates the BMP signaling pathway by binding to BMP proteins and preventing them from binding to receptors. As an antagonist of BMPs, Noggin prevents chondrocyte proliferation and thus regulates the normal formation of joints. When culturing human embryonic stem cells (hESCs) or neural stem cells under certain conditions, adding Noggin to antagonize BMP activity can allow stem cells to proliferate while maintaining their undifferentiated state.

Storage

Store below -20°C

Validity period

24 months

References

1. Messina A, Incitti T, Bozza A, Bozzi Y, Casarosa S. Noggin Expression in the Adult Retina Suggests a Conserved Role during Vertebrate Evolution. J Histochem Cytochem. 2014 Jul;62(7):532-40. doi: 10.1369/0022155414534691.

2. Krause C, Guzman A, Knaus P. Noggin. Int J Biochem Cell Biol. 2011 Apr;43(4):478-81. doi: 10.1016/j.biocel.2011.01.007. Epub 2011 Jan 21.

3. Wang Y, Zheng Y, Chen D, Chen Y. Enhanced BMP signaling prevents degeneration and leads to endochondral ossification of Meckel's cartilage in mice. Dev Biol. 2013 Sep 15;381(2):301-11. doi: 10.1016/j.ydbio.2013.07.016. Epub 2013 Jul twenty three.

4. Sui L, Geens M, Sermon K, Bouwens L, Mfopou JK. Role of BMP signaling in pancreatic progenitor differentiation from human embryonic stem cells. Stem Cell Rev Rep. 2013 Oct;9(5):569-77. doi: 10.1007/s12015-013-9435-6.

Precautions

This product is only suitable for in vitro cell culture and cannot be used directly in clinical treatment.

Documents

Recombinant human Noggin protein instructions

COA

SDS

Recombinant Human Noggin Protein