Description

The recombinant human leukemia inhibitory factor (LIF) protein is transiently expressed in HEK-293 cells and features a C-terminal His-tag for purification.

As a pleiotropic IL-6 family cytokine, LIF signals through the LIFR-α/gp130 receptor complex, activating the JAK/STAT pathway.

Its core applications in cell and gene therapy include maintaining pluripotency and suppressing spontaneous differentiation of mouse embryonic stem cells (mESCs) for in vitro expansion culture, and promoting the differentiation of neural progenitor cells into cholinergic neurons.

Designed to preserve native bioactivity, this protein is suited for regenerative medicine applications such as stem cell therapy and neural repair.

Specifications

Product Details
Synonyms	CDF, DIA, HILDA, Leukemia Inhibitory Factor, MLPLI
Accession	UniProt P15018
Expressed Region	Ser23–Phe202
Tag	C-terminal 6×His-tag
Expression System	HEK293 cells
Predicted Molecular Weight	20.5 kDa
Purity	> 95% as determined by SDS-PAGE and HPLC
Endotoxin	< 0.01 EU per 1 μg of protein (LAL method)
Formulation	Lyophilized from sterile PBS (pH 7.4), typically supplemented with 6% mannitol as a stabilizing protectant

Features

✔ High Purity: Purified using advanced chromatographic techniques, this product achieves a purity level of over 95%, ensuring accurate and reproducible experimental results.
✔ High Biological Activity: Validated through multiple activity assays, this LIF protein effectively promotes cell differentiation and survival, making it suitable for a range of research applications.
✔ Batch Consistency: Produced under stringent quality control measures to ensure high consistency between batches, reducing variability and enhancing experimental reliability.
✔ Animal-Free: Manufactured without the use of animal-derived components, adhering to animal-free standards and broadening its applicability for diverse research needs.

Applications

Stem Cell Research

Study the role of LIF in maintaining the pluripotency of embryonic stem cells and promoting their differentiation into various cell lineages, advancing research in regenerative medicine.

Immunology

Investigate the effects of LIF on immune cell function and differentiation, providing insights into immune responses and potential therapeutic applications.

Oncology

Explore the role of LIF in cancer cell biology, including its impact on tumor growth and metastasis, contributing to the development of novel cancer therapies.

Hematopoiesis

Utilize LIF to study its role in the regulation of hematopoietic stem cells and progenitor cells, offering valuable information for the treatment of hematological disorders.

Stability & Storage

√ Lyophilized powder: Stable for 12 months at -80°C or 6 months at -20°C when stored in the original sealed container under desiccant.
√ Reconstitution: Dissolve in sterile water for injection, 0.9% NaCl, or PBS (pH7.4), maintaining a final concentration ≥ 100 µg/mL to prevent adsorption.
√ Handling: Aliquot to avoid repeated freeze-thaw cycles.

Cautions

• Research Use Only: This product is not intended for diagnostic or therapeutic use.

• Handling: Follow recommended storage and handling instructions to maintain product stability.

• Support: Contact technical support if assistance is required.

Document

📄 GMP-TL633 Data Sheet 📄 GMP-TL633 SDS

Recombinant Human LIF Protein