Background

LIF is a multitrophic factor produced by a variety of cell types, including T cells, myeloid cell lines, fibroblasts, liver, heart, and melanoma. LIF promotes the long-term maintenance of embryonic stem cells by inhibiting the spontaneous differentiation of embryonic stem cells. Other activities include stimulation of acute time-phase protein synthesis by hepatocytes, stinging differentiation of kincholinergic nerves, as well as inhibition of lipogenesis by inhibition of lipoprotein lipase in fat cells. Human LIF is active on mouse cells, being widely used to maintain spontaneous differentiation of mouse ESC, which does not work on human cells due to their inability to bind to human LIF receptors. Recombination Human LIF contains 180 amino acids with a molecular weight of 19.6 KDa, containing 3 disulfide bonds.

Specifications

Protein sequence	A DNA sequence encoding Human LIF (NP_002300.1) is expressed with His tag at the C-terminal
Expression Host	HEK293 cells
QC Testing Purity	≥95 % as determined by SDS-PAGE and HPLC analyses
Activity	Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED₅₀ is 0.1 ng/ml, corresponding to a specific activity of 1 x 10⁷ units/mg
Endotoxin Level	≤0.01 EU per µg of the protein as determined by the LAL method
Molecular Mass	Recombinant human LIF is a 20.5 kD protein consisting of 186 amino acid residues
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization
Stability & Storage	24 months at 2 °C to 8 °C in lyophilized state. 6-12 months at -20 °C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.

References

Fast high-throughput screening of glutathione S-transferase polymorphism by voltage programming-based multi-channel microchip electrophoresis.Sun Y, Kim SK, Kang SH.J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Sep 1;1097-1098:10-17. doi: 10.1016/j.jchromb.2018.08.034.
Plant Growth Modulates Metabolites and Biological Activities in Retama raetam (Forssk.) Webb.Saada M, Falleh H,Catarino MD, Cardoso SM, Ksouri R.Molecules. 2018 Aug 29;23(9). pii: E2177. doi: 10.3390/molecules23092177.
A Zombie LIF Gene in Elephants Is Upregulated by TP53 to Induce Apoptosis in Response to DNA Damage.Vazquez JM, Sulak M, Chigurupati S, Lynch VJ.Cell Rep. 2018 Aug 14;24(7):1765-1776. doi: 10.1016/j.celrep.2018.07.042.
Integrative transcriptome and microRNome analysis identifies dysregulated pathways in human Sertoli cells exposed to TCDD.Ribeiro MA, Estill MS, Fernandez GJ, Moraes LN, Krawetz SA, Scarano WR.Toxicology. 2018 Aug 7;409:112-118. doi: 10.1016/j.tox.2018.08.001.

Document

GMP-TL633

GMP-TL633_SDS

Recombinant Human LIF Protein