Product Information

Protein sequence	A DNA sequence encoding the human IL-3 (GenBank: AAA59146.1) was expressed with a His-tag at the C-terminus
Expression Host	HEK293 cells
QC Testing Purity	> 90 % as determined by SDS-PAGE
Activity	Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is ≤ 5 ng/mL
Endotoxin Level	< 0.1 EU per 1 μg of the protein by the LAL method
Molecular Mass	The recombinant human IL-3 protein consists of 139 amino acids and predicts a molecular mass of 15.9 kD
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization
Stability & Storage	24 months at 2℃ to 8℃ in lyophilized state. 6 months at -20℃ under sterile conditions after reconstitution. 12 months at -80℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100 μg/mL. Avoid repeated freeze-thaw cycles.

Background

IL-3 is a hematopoietic growth factor that promotes the survival, differentiation, and proliferation of megakaryocytes, granulocyte-macrophages, erythrocytes, eosinophils, basophils, and progenitors of the mast cell line. IL-3 is produced by T cells, mast cells, and eosinophils and enhances platelet production, phagocytosis, and antibody-mediated cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunity epidemic regulation. Much of the activity of IL-3 depends on costimulation with other cytokines. IL-3 is a species-specific, mutable glycosylated cytokine.

References

Loss of solute carrier family 7 member 2 exacerbates inflammation-associated colon tumorigenesis. Coburn LA, Singh K, Asim M, Barry DP, Allaman MM, Al-Greene NT, Hardbower DM, Polosukhina D, Williams CS, Delgado AG, Piazuelo MB, Washington MK, Gobert AP, Wilson KT. Oncogene. 2018 Sep 10. doi: 10.1038/s41388-018-0492-9.
Irreversible effects of trichloroethylene on the gut microbial community and gut-associated immune responses in auto immune-prone mice. Khare S, Gokulan K, Williams K, Bai S, Gilbert KM, Blossom SJ.J Appl Toxicol. 2018 Sep 5. doi 10.1002/jat.3708.
Comparative utility of NRG and NRGS mice for the study of normal hematopoiesis, leukemogenesis, and therapeutic response.Barve A, Casson L, Krem M, Wunderlich M, Mulloy JC, Beverly LJ. Exp Hematol. 2018 Aug 17. pii: S0301-4 72X(18)30751-3. doi: 10.1016/j.exphem.2018.08.004.

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Recombinant Human IL-3 Protein

Recombinant Human IL-3 Protein_SDS