Description

Recombinant Human Interleukin-2 (IL-2) is a cytokine that plays a central role in the regulation of immune responses. It is primarily produced by activated T lymphocytes and promotes the proliferation, differentiation, and survival of T cells, B cells, and natural killer (NK) cells. This recombinant IL-2 protein is expressed in CHO cells and corresponds to the mature human IL-2 sequence (Ala21–Thr153), ensuring proper folding and post-translational modifications. With high purity (≥95%) and strong bioactivity (≥1 × 10⁷ IU/mg), this product is suitable for a wide range of immunological and cell biology studies. The protein is supplied in a tag-free, lyophilized form, making it convenient for reconstitution and downstream applications.

Specifications

Product Details
Cat. No.	Y02001N
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤1 EU/mg by the LAL method.
Bioactivity	≥1 x 10⁷ IU/mg. Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells.
Accession	P60568-1
Species	Human
Expression System	CHO
Mol. Weight	15.4 kDa
Expression Region	Ala21-Thr153
Tag Info	Tag free
Formulation	Lyophilized
Buffer	PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	100μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: High Purity: This product undergoes stringent purification processes, achieving a purity level of ≥95%, ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Exhibits a bioactivity of ≥1 × 10⁷ IU/mg, validated by CTLL-2 cell proliferation assay, ensuring strong and consistent performance in functional studies.
✔ Mammalian Expression System: Expressed in CHO cells to ensure proper protein folding and native post-translational modifications, enhancing biological relevance.
✔ Low Endotoxin: Endotoxin level is ≤1 EU/mg, making it suitable for sensitive cell-based assays and immune-related studies.

Applications

Hematology

Regulates hematopoietic balance under inflammation, favoring myeloid differentiation.

Immunology

Potent pro-inflammatory cytokine driving innate immunity and Th17 responses.

Cell Biology

Participates in inflammation signaling, cell activation, and death regulation.

Data Figures

Measured by its ability to induce NF-kB reporter activity in MCF-7 cells.

Figure 1: Recombinant Human IL-2 (Cat. No. Y02001) stimulates proliferation of CTLL-2 cells. The specific bioactivity of Y02001 is ≥1 x 10⁷ IU/mg.

Figure 2: Recombinant Human IL-2 (Cat. No. Y02001) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y02001 SDS-PAGE.

Figure 3: Highly consistent bioactivity between different production batches of Recombinant Human IL-2 (Cat. No. Y02001).

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.