Description

IL-1β (Interleukin-1β) is a key pro-inflammatory cytokine that plays a central role in immune regulation, inflammatory responses, and cellular signaling. The mature form of IL-1β adopts a β-trefoil structure composed of 12 β-strands and interacts via surface residues with the IL-1R1 receptor to initiate downstream signaling. IL-1β stimulates the secretion of other inflammatory factors, such as TNF-α and IL-6, as well as chemokines, thereby mimicking an in vivo inflammatory environment. Additionally, it activates immune cells by promoting T cell proliferation, B cell differentiation, and enhancing the antigen-presenting capacity of macrophages and dendritic cells.

Specifications

Product Details
Cat. No.	Y04921H
Purity	≥95%, by SDS-PAGE (under reducing (R)& Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤100 EU/mg by the LAL method.
Bioactivity	Measured by its ability to induce NF-kB reporter activity in MCF-7 cells. The ED50 is ≤10 pg/mL.
Accession	P01584
Species	Human
Expression System	E. coli
Mol. Weight	17.4 kDa
Expression Region	Ala117-Ser269
Tag Info	Tag free
Formulation	Lyophilized
Buffer	PB, 5% Trehalose and 0.01% Tween 80, pH7.4.
Size	50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Regulates hematopoietic balance under inflammation, favoring myeloid differentiation.

Immunology

Potent pro-inflammatory cytokine driving innate immunity and Th17 responses.

Cell Biology

Participates in inflammation signaling, cell activation, and death regulation.

Data Figures

Figure 1: Measured by its ability to induce NF-kB reporter activity in MCF-7 cells.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Recombinant Human IL-1B