Description

IL-17A (Interleukin-17A) is a pro-inflammatory cytokine mainly produced by Th17 cells, as well as γδ T cells and innate lymphoid cells. It signals through the IL-17RA/IL-17RC receptor complex, activating NF-κB and MAPK pathways to induce inflammatory responses. Functionally, IL-17A promotes chemokine and cytokine production, drives neutrophil recruitment, and plays a key role in mucosal defense against extracellular bacteria and fungi. In in vitro cell and organoid models, IL-17A is commonly used to mimic inflammatory microenvironments and often acts synergistically with TNF-α or IL-22. Dysregulated IL-17A signaling is implicated in autoimmune and chronic inflammatory diseases such as psoriasis and inflammatory bowel disease.

Specifications

Product Details
Cat. No.	Y06601H
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is＜10 ng/mL.
Accession	Q16552
Species	Human
Expression System	CHO
Mol. Weight	15.5 kDa
Expression Region	Ile20-Ala155
Tag Info	Tag free
Form	Lyophilized
Buffer	Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% Mannitol, 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to-80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Involved in inflammation-related hematopoietic regulation.

Immunology

Signature Th17 cytokine driving chronic inflammation.

Cell Biology

Induces expression of inflammation-related genes.

Data Figures

Figure 1: Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is＜10 ng/mL.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA

Recombinant Human IL-17A