Description

IL-12 (Interleukin-12) is a key pro-inflammatory cytokine and a member of the IL-12 family, primarily produced by antigen-presenting cells such as dendritic cells, macrophages, and B cells. It is a heterodimeric protein (p70) composed of two subunits, p35 and p40, linked by a disulfide bond. IL-12 promotes Th1 cell differentiation by activating the STAT4 signaling pathway, driving the commitment of naïve CD4⁺ T cells into Th1 cells and stimulating the secretion of pro-inflammatory cytokines such as IFN-γ. It also enhances the activity of NK and T cells by promoting the proliferation and cytotoxic function of NK cells and CTLs (cytotoxic T lymphocytes). Furthermore, IL-12 suppresses Th2 responses by inhibiting IL-4-mediated Th2 cell differentiation, thereby reinforcing cell-mediated immunity.

Specifications

Product Details
Cat. No.	Y00401
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is ＜0.5 ng/mL.
Accession	P29460(IL12P40), P29459(IL12P35)
Alternative Names	Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; NK cell; IL12A ; NKSF1 stimulatory factor chain 1
Species	Human
Expression System	CHO
Mol. Weight	59 kDa
Expression Region	Ile23-Ser328&Arg23-Ser219
Tag Info	Tag free
Form	Lyophilized
Buffer	PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at 2°C to 8°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Influences maturation of NK and T cell functions.

Immunology

Induces Th1 differentiation and enhances cytotoxic immunity.

Cell Biology

Enhances cytotoxicity-related signaling and effector functions.

Data Figures

Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is ＜0.5 ng/mL.

Figure 1: Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is ＜0.5 ng/mL.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA

Recombinant Human IL-12