Description

IFN-γ (Interferon-gamma) is a cytokine produced by immune cells such as T cells and NK cells, belonging to the type II interferon family. It plays a central role in immune regulation, antiviral and antitumor responses, and immune defense. IFN-γ induces the production of antiviral proteins (e.g., PKR, OAS) that inhibit viral replication and enhances the phagocytic and pathogen-killing capacity of macrophages. In terms of cell proliferation and apoptosis, IFN-γ suppresses the growth of certain tumor cells and promotes their apoptosis. In stem cell culture, it inhibits the adipogenic differentiation of mesenchymal stem cells (MSCs) while promoting osteogenic differentiation.

Specifications

Product Details
Cat. No.	Y01801
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a cell proliferation assay using Mo7e human leukemia cell line. The ED50 for this effect is ≤ 2 ng/mL.
Accession	P01579
Alternative Names	Interferon Gamma; IFN-Gamma; Immune Interferon; IFNG
Species	Human
Expression System	CHO
Mol. Weight	16.8 kDa
Expression Region	Gln24-Gln166
Tag Info	Tag free
Formulation	Lyophilized
Buffer	PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Modulates hematopoietic progenitor differentiation under inflammatory conditions.

Immunology

Key cytokine for Th1 responses, activates macrophages and NK cells.

Stem Cell Research

Used to stimulate immune-like differentiation in stem cell cultures.

Cell Biology

Regulates cell proliferation, apoptosis, and immune signaling.

Data Figures

Figure 1: Recombinant Human IFNγ (Cat. No. Y01801) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y01801 SDS-PAGE.

Recombinant Human IFNγ (Cat. No. Y01801) stimulates proliferation of Mo7e cells. The ED50 for this effect is ≤2 ng/mL. The bioactivity of Y01801 was higher than the other competing product.

Figure 2: Recombinant Human IFNγ (Cat. No. Y01801) stimulates proliferation of Mo7e cells. The ED50 for this effect is ≤2 ng/mL. The bioactivity of Y01801 was higher than the other competing product.

Figure 3: Highly consistent bioactivity between different production batches of Recombinant Human Transferrin (Cat. No. Y01801).

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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