Description

Recombinant Human Holo-Transferrin (HOLO) is an iron-saturated form of transferrin, a key glycoprotein responsible for iron transport and delivery in biological systems. Unlike apo-transferrin, holo-transferrin is fully loaded with iron, enabling efficient cellular iron uptake via transferrin receptor-mediated endocytosis. It plays a critical role in supporting cell proliferation, metabolism, and viability, especially in serum-free and chemically defined culture systems.

This recombinant protein is expressed in CHO cells and corresponds to the full-length human transferrin sequence (Val20–Pro698), ensuring proper folding and biological functionality. With high purity (≥95%) and low endotoxin levels (≤0.5 EU/mg), this product provides consistent performance in sensitive cell culture applications. Supplied in a tag-free, lyophilized form, it is ideal for large-scale cell culture, bioprocessing, and research use.

Specifications

Product Details
Cat. No.	Y04501
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤0.5 EU/mg by the LAL method.
Bioactivity	Measured in a serum-free cell proliferation assay using MDCK canine kidney epithelial cells. The ED50 for this effect is 0.5-2 μg/mL. Optimal concentration depends on cell type as well as the application or research objectives.
Accession	P02787
Alternative Names	Serotransferrin; Transferrin; Beta-1 metal-binding globulin; Siderophilin; TF
Species	Human
Expression System	CHO
Mol. Weight	75.2 kDa
Expression Region	Val20-Pro698
Tag Info	Tag free
Formulation	Lyophilized
Buffer	Sterile water
Size	10μg/100μg/1mg
Lead Time	Products are in stock,but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of ≥95%, ensuring reliable and reproducible experimental results.
✔ Low Endotoxin: Endotoxin level is ≤0.5 EU/mg, making it suitable for sensitive cell culture and biomanufacturing applications.
✔ Iron-Saturated Form: Provided as holo-transferrin, enabling efficient iron delivery and enhanced support for cell growth and metabolism.
✔ Optimized for Serum-Free Culture: Ideal for use in chemically defined and serum-free media, ensuring consistent and controlled experimental conditions.

Applications

Cell Culture

Widely used in serum-free and chemically defined media to support cell growth, viability, and proliferation.

Bioprocessing

Suitable for large-scale cell culture and biopharmaceutical production requiring consistent iron supplementation.

Cell Biology

Used to study iron metabolism, transferrin receptor function, and iron-dependent cellular processes.

Data Figures

Measured in a cell inhibitionn assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.2 ng/mL. The bioactivity of Y07901 was higher than other competing products.

Figure 1: Recombinant Human Transferrin (Cat. No. Y04501) SDS-PAGE under reducing (R) & Non-reducing condition. The gel was stained with Y04501 SDS-PAGE.

Figure 2: Recombinant Human Transferrin (Cat. No. Y04501) stimulates proliferation of MDCK cells in serum-free cell culture medium. The ED50 for this effect is 0.5-2 μg/mL. The bioactivity of Y04501 was higher than other competing products.

Figure 3: Highly consistent bioactivity between different production batches of Recombinant Human Transferrin (Cat. No. Y04501).

Figure 4: SEC-HPLC

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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