Description

Glial cell line-derived neurotrophic factor (GDNF) is a neurotrophic factor belonging to the transforming growth factor-beta (TGF-β) superfamily.

It is expressed in various brain regions, including the hippocampus, amygdala, cerebral cortex, and cerebellum, and is involved in the growth and morphological regulation of dopaminergic neuronal projections.

GDNF supports the survival and maintenance of several neuronal populations, including sensory neurons, retinal ganglion cells, cholinergic neurons, and spinal motor neurons, making it a potential therapeutic agent for neurodegenerative diseases.

Specifications

Parameter	Specification
Expression Host	E. coli
Synonyms	Glial cell line-derived neurotrophic factor; hGDNF; Astrocyte-derived trophic factor (ATF)
Protein Sequence	Amino acid sequence: Ser78–Ile211 of human GDNF (UniProt ID: P39905-1)
Molecular Weight	Predicted molecular weight is 15.06 kDa
Purity	>95%, analyzed by SDS-PAGE gel and HPLC
Endotoxin	<0.1 EU/μg
Purification Method	Chromatographic purification
Appearance	White lyophilized powder
Activity	Refer to COA for details

Features

✔ E. coli–Expressed Human GDNF (Ser78–Ile211)
Recombinant human GDNF corresponding to Ser78–Ile211 expressed in E. coli, with a predicted molecular weight of 15.06 kDa.
✔ High Purity Chromatographic Preparation
Purity >95% verified by SDS-PAGE gel and HPLC following chromatographic purification.
✔ Low Endotoxin Profile
Endotoxin level <0.1 EU/μg suitable for sensitive in vitro cell culture systems.
✔ Defined Lyophilized Protein Format
Supplied as a white lyophilized powder with verified electrophoretic identity and consistent protein quality.

Applications

Neurotrophic Factor Research

Supports studies on neuronal survival, maintenance, and development of dopaminergic neuronal projections.

Neurodegenerative Disease Models

Applied in research involving neuronal populations such as sensory neurons, retinal ganglion cells, cholinergic neurons, and spinal motor neurons.

Stem Cell Culture Studies

Used in stem cell-related experiments including MSC and iPSC culture systems.

Neuronal Differentiation Studies

Facilitates investigations into neuronal growth and morphological regulation in neural cell culture models.

Storage

Store below -20°C

Instructions

Centrifuge before reopening the tube. Dissolve the lyophilized protein in distilled water. It is recommended to reconstitute the concentration to greater than 100 μg /mL. After reconstitution, the protein can be stored at -80°C for 3-6 months and at 2-8℃ for 2-7 days. For optimal storage, aliquot the protein and minimize freeze-thaw cycles.

Precautions

This product is only suitable for in vitro cell culture and cannot be used directly in clinical treatment.

Documents

📄 TL-771 Data Sheet 📄 TL-771 SDS 📄 TL-771 COA

Recombinant Human GDNF Protein