Description

Recombinant Human Granulocyte Colony-Stimulating Factor (G-CSF) is a key hematopoietic cytokine that regulates the proliferation, differentiation, and activation of neutrophilic granulocyte precursors. It functions by binding to the G-CSF receptor (CSF3R), activating downstream signaling pathways such as JAK/STAT, PI3K/AKT, and MAPK, thereby promoting neutrophil production and survival. G-CSF plays a critical role in immune response and hematopoietic recovery.

This recombinant protein is expressed in CHO cells and corresponds to the mature human G-CSF sequence (Thr31–Pro204), ensuring proper folding and biological activity. With high purity (≥95%) and strong bioactivity (ED50 ＜0.5 ng/mL; ≥1 × 10⁷ units/mg in NFS-60 cell proliferation assay), this product is suitable for research in hematology, immunology, and cell biology. The protein is supplied in a tag-free, lyophilized form for convenient storage and reconstitution.

Specifications

Product Details
Cat. No.	Y06201
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	The bioactivity is determined by the dose-dependent proliferation of murine NFS-60 cells is ＜ 0.5 ng/mL, corresponding to a specific bioactivity of ≥ 1 x 107 units/mg.
Accession	P09919
Alternative Names	Granulocyte Colony-Stimulating Factor; G-CSF; Pluripoietin; Filgrastim; Lenograstim; CSF3; C17orf33; GCSF
Species	Human
Expression System	CHO
Mol. Weight	18.7 kDa
Expression Region	Thr31-Pro204
Tag Info	Tag free
Formulation	Lyophilized
Buffer	PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of ≥95%, ensuring reliable and reproducible experimental results.
✔ High Biological Activity: Exhibits potent activity with ED50 ＜0.5 ng/mL and ≥1 × 10⁷ units/mg, validated in NFS-60 cell proliferation assay.
✔ Mammalian Expression System: Expressed in CHO cells to ensure proper folding and native-like biological functionality.
✔ Convenient and Reliable: Supplied in a tag-free, lyophilized form for easy reconstitution, stable storage, and compatibility with diverse experimental applications.

Applications

Hematology

Promotes proliferation and differentiation of neutrophil precursors, widely used in hematopoietic studies.

Immunology

Enhances innate immune responses through regulation of neutrophil production and function.

Cell Biology

Used to study cytokine signaling pathways such as JAK/STAT and mechanisms of cell proliferation and survival.

Data Figures

Measured in a cell inhibitionn assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.2 ng/mL. The bioactivity of Y07901 was higher than other competing products.

Figure 1: The activity is determined by the dose-dependent proliferation of murine NFS-60 cells is ＜0.5 ng/mL, corresponding to a specific activity of ≥ 1 x 10⁷ units/mg.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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Recombinant Human G-CSF