Description

FGF-4 (Fibroblast Growth Factor 4), a member of the FGF family, is a secreted glycoprotein that activates downstream signaling pathways (such as MAPK and PI3K-AKT) by binding to its receptors (FGFRs). It promotes cell proliferation, differentiation, migration, and survival, playing a central role in embryonic development, mesoderm formation, and stem cell maintenance. In stem cells, it helps maintain pluripotency; for example, FGF-4 and LIF (Leukemia Inhibitory Factor) act synergistically to sustain the undifferentiated state of mouse embryonic stem cells (ESCs). Additionally, FGF-4 can promote the differentiation of specific cell types and is used in the culture of primary cells.

Specifications

Product Details
Cat. No.	Y00921H
Purity	≥95%, by SDS-PAGE (under conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.25-1 ng/mL.
Accssion	P08620
Alternative Names	Fibroblast growth factor 4; FGF-4; Heparin secretory-transforming protein 1; HST; HST-1; HSTF-1; Heparin-binding growth factor 4; HBGF-4; Transforming protein KS3; FGF4; HST; HSTF1; KS3
Species	Human
Expression System	E. coli
Mol. Weight	19.3 kDa
Expression Region	Ala31-Leu206
Tag Info	Tag free
Formulation	Lyophilized
Buffer	PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Stem Cell Research

Supports ESC proliferation and survival, especially in combination with FGF-2 for pluripotent stem cells.

Cell Biology

Involved in early embryonic development signaling and cell proliferation.

Data Figures

Figure 1: Recombinant Human FGF4 (Cat. No. Y00901) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y00901 SDS-PAGE.

Recombinant Human FGF4 (Cat. No. Y00901) stimulates proliferation of NIH3T3 cells. The ED50 for this effect is 0.25-1 ng/mL. The bioactivity of Y00901 was higher than the other competing product.

Figure 2: Recombinant Human FGF4 (Cat. No. Y00901) stimulates proliferation of NIH3T3 cells. The ED50 for this effect is 0.25-1 ng/mL. The bioactivity of Y00901 was higher than the other competing product.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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Recombinant Human FGF-4