Description

FGF-2 (Fibroblast Growth Factor-2 (Basic)), also known as bFGF, is one of the earliest recognized members of the FGF family. Secreted by endothelial cells, smooth muscle cells, and macrophages, FGF-2 acts on a variety of cell types. As a crucial regulator of cell growth and differentiation, FGF-2 possesses biological activities such as pro-angiogenic, proliferative, chemotactic, and migratory effects, playing a vital role in processes of cell differentiation and organismal development.

Specifications

Product Details
Cat. No.	Y00351H
Purity	≥95%, by SDS-PAGE (under reducing (R)&Non-reducing conditions,visualized by Coomassie staining).
Endotoxin	≤20 EU/mg by the LAL method.
Bioactivity	Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast Cells. The ED50 for this effect ≤2.0 ng/mL.
Accession	P09038-4
Species	Human
Expression System	E. coli
Mol. Weight	16.5 kDa
Expression Region	Pro143-Ser288
Tag Info	Tag free
Form	Lyophilized
Buffer	Lyophilized from a 0.2 μm filtered solution containing PB, 5% Trehalose and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Supports angiogenesis in hematopoietic niches, indirectly influencing hematopoiesis.

Immunology

Modulates immune cell proliferation and tissue repair indirectly.

Stem Cell Research

Widely used to maintain pluripotency and promote proliferation of ESCs and iPSCs.

Cell Biology

Key regulator of proliferation, survival, migration, and differentiation.

Data Figures

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA

Recombinant Human FGF-2