Description

EPO (Erythropoietin), also known as erythrocyte-stimulating factor, is an endogenous glycoprotein hormone composed of 165 amino acids. During the fetal period, EPO is primarily produced in the liver, while after birth its synthesis occurs mainly in the kidneys. Additionally, the liver, brain, reproductive system, bone marrow macrophages, and mammary glands also have the capacity to produce EPO autonomously. Its synthesis is regulated by hypoxia-inducible factors (HIFs), among other mechanisms. As an important cytokine in humans, the most well-known and essential biological function of EPO is to stimulate erythrocyte proliferation and promote hematopoiesis. Beyond its role in blood formation, EPO also exhibits other effects, such as inhibiting pro-inflammatory cytokines, protecting cells from apoptosis, and promoting angiogenesis in both normal and tumor tissues.

Specifications

Product Details
Cat. No.	Y05801
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is ≤0.1 ng/mL.
Accession	P01588
Alternative Names	Erythropoietin; Epoetin; EPO
Species	Human
Expression System	CHO
Mol. Weight	18.3 kDa
Expression Region	Ala28-Arg193
Tag Info	Tag free
Formulation	Lyophilized
Buffer	Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Stimulates erythroid progenitor proliferation and differentiation.

Immunology

Modulates immune cell function and inflammation indirectly.

Stem Cell Research

Used to promote hematopoietic lineage commitment in stem cell cultures.

Cell Biology

Regulates erythropoiesis and cell survival via JAK2/STAT5 signaling.

Data Figures

Figure 1: Recombinant Human EPO (Cat. No. Y05801) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y05801 SDS-PAGE.

Figure 2: ‍Recombinant Human EPO (Cat. No.Y05801) stimulates proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is ≤0.1 ng/mL.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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