Description

Recombinant Human Epidermal Growth Factor (EGF) is a crucial protein involved in the regulation of cell growth, proliferation, and differentiation. EGF binds to its receptor EGFR, initiating a cascade of downstream signaling pathways that influence cellular processes, making it an indispensable tool in research areas such as cell biology, wound healing, and oncology.

Features

High Purity: This product is subjected to rigorous purification processes, achieving a purity level of over 95%, ensuring the reliability and reproducibility of experimental results.
High Biological Activity: Multiple activity assays confirm that this product effectively stimulates the expected biological responses in target cells, suitable for a wide range of research applications.
Batch Consistency: Manufactured under strict production and quality control protocols to ensure high consistency between batches, reducing variability in experimental outcomes.
Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements and broadening its application scope in research.

Applications

Cell Biology: Used to study cellular processes such as proliferation, differentiation, and survival, EGF is crucial in understanding fundamental cell biology.
Wound Healing: EGF promotes the regeneration of epidermal tissues, making it a valuable component in research focused on wound healing and tissue repair.
Oncology: Investigates the role of EGF in cancer cell proliferation and survival, providing insights into potential therapeutic targets for cancer treatment.
Regenerative Medicine: Utilized in research aiming to develop therapies for tissue regeneration and repair, contributing to advances in regenerative medicine.

Specifications

Cytokines
Expression Host	HEK293 cells
Molecular Mass	Predicts a molecular mass of 7.0 kD.
QC Testing Purity	> 90 % as determined by SDS-PAGE.
Endotoxin	< 0.1EU per 1 μg of the protein by the LAL method.
Activity	Measured in a cell proliferation assay using Balb/c 3T3 cells. The ED₅₀ for this effect is ≤3 ng/mL.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.
Stability	24 months at 2℃ to 8℃ in lyophilized state. 6 months at -20℃ under sterile conditions after reconstitution. 12 months at -80℃ under sterile conditions after reconstitution.
Storage	Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100 μg/mL. Avoid repeated freeze-thaw cycles.

Data Figures

Storage and Stability

Packaging: Supplied in sterile lyophilized form for ease of storage and transport.
Storage: Store at -20°C or below to maintain product stability and activity. Avoid repeated freeze-thaw cycles to prevent protein degradation.
Stability: Stable for long-term storage under recommended conditions, ensuring reliable performance in research applications.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

This product is intended for research use only and is not suitable for clinical diagnostics or therapeutic applications.
Handle the product according to provided instructions to preserve its activity and stability.
Contact our technical support team if you encounter any issues or have questions regarding the product's use.

Documents

GMP-TL613_SDS.pdf

Recombinant Human EGF Protein