Description

CNTF (Ciliary Neurotrophic Factor) is a neurotrophic factor structurally similar to IL-6 and LIF, featuring a characteristic four-helix bundle topology. It was initially identified based on its ability to support neuronal survival, particularly in the ciliary ganglion. CNTF promotes the survival of various types of neurons, including sympathetic, sensory, motor, and hippocampal neurons. It also induces the proliferation and differentiation of astrocytes and stimulates the secretion of other neurotrophic factors. Furthermore, when used in combination with EGF/bFGF, CNTF can induce the differentiation of neural stem cells into neurons or glial cells.

Specifications

Product Details
Cat. No.	Y05621H
Purity	≥95%
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a cell proliferation assay using TF-1 cells. The ED50 is ＜100 pg/ml.
Accession	NP_000605.1
Species	Human
Expression System	E. coli
Mol. Weight	22.9 kDa
Expression Region	M1-M200
Tag Info	Tag free
Formulation	Lyophilized
Buffer	Lyophilized from a 0.22 μm-filtered solution containing 20mM Citric acid, 5% Mannitol and 0.01% Tween 80, pH2.5.
Size	10μg/50μg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Stem Cell Research

Promotes neural stem/progenitor cell survival and differentiation.

Cell Biology

Enhances neuronal survival and differentiation through JAK/STAT signaling.

Data Figures

Figure 1: Measured in a cell proliferation assay using TF-1 cells.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA

Recombinant Human CNTF