Description

BMP-4 is a disulfide-stabilized homodimeric growth factor and core member of the Bone Morphogenetic Protein (BMP) subfamily within the TGF-β superfamily. It specifically binds type I (BMPR1A) and type II (BMPR2) receptors to activate SMAD1/5/8-SMAD4 signaling, precisely governing mesodermal lineage commitment, neurogenesis, and angiogenesis.

This protein induces osteogenic differentiation of mesenchymal stem cells via Runx2/Osterix transcriptional programs and coordinates with PTHRP to regulate mammary ductal morphogenesis and hair follicle suppression. Its activity is tightly modulated by extracellular antagonists (e.g., Noggin/Chordin), with dysregulation linked to pathologies like Fibrodysplasia Ossificans Progressiva (FOP).

For cell therapy applications, BMP-4 serves as a pivotal differentiation inducer with dual functions:

Temporal gradient dosing in 3D hiPSC cultures efficiently generates mesodermal progenitors for cardiac regenerative therapies;
Synergy with ROCK inhibitors to engineer vascular networks in organoids, enhancing in vivo integration and vascularization of osteochondral grafts.

Produced via mammalian cell transient transfection and chromatographic purification, this product retains native dimeric conformation and full bioactivity.

Specifications

Specification	Acceptance Criteria	Results
Appearance	Freeze-dried preparation; white, loose solid	Compliance
Identity	The electrophoretic bands should be consistent with the molecular weight of the product	Compliance
Visible Particles	No visible particles	Compliance
Moisture	≤ 4.0%	2.0%
Purity	> 90.0%	Compliance
Endotoxin	< 0.1 EU/μg	< 0.1 EU/μg
Mycoplasma	Negative	Negative
Sterility	No growth	No growth
Biological Activity	Induction of alkaline phosphatase production in ATDC-5 cells; ED₅₀ ≤ 100 ng/mL	ED₅₀ = 12.04 ng/mL

Features

✔ ative Dimeric BMP-4 Activity
Disulfide-stabilized homodimer preserves full BMPR-SMAD1/5/8 signaling fidelity.
✔ High Differentiation Potency
Strong osteo- and mesoderm-inducing activity validated by ATDC-5 ALP assay (ED₅₀ ≈ 12 ng/mL).
✔ Mammalian Expression, Tag-Free
HEK293 production ensures proper folding and bioactivity without tag-related interference.
✔ Excellent Batch Consistency
Tight QC on purity, endotoxin, sterility, and mycoplasma supports reproducible differentiation outcomes.

Applications

MSC Osteogenic Differentiation

Drives Runx2/Osterix programs for bone and osteochondral tissue engineering.

iPSC Mesoderm Induction

Enables controlled generation of mesodermal progenitors for cardiac and musculoskeletal models.

Organoid & 3D Culture Systems

Synergizes with ROCK inhibitors to promote vascularized organoid formation.

Regenerative Medicine Research

Supports studies in bone repair, developmental biology, and lineage specification.

Stability & Storage

Lyophilized powder: Store at −20 °C in the original sealed container to maintain stability.
Reconstitution: Reconstitute with sterile Water for Injection, 0.9% NaCl, or PBS (pH 7.4) prior to use.
Handling: After reconstitution, aliquot the solution and avoid repeated freeze–thaw cycles.

Cautions

• Research Use Only: This product is not intended for diagnostic or therapeutic use.

• Handling: Follow recommended storage and handling instructions to maintain product stability.

• Safety: Use appropriate PPE and standard laboratory safety practices when handling this product.

Documents

📄 TL-607 COA 📄 TL-607 SDS 📄 TL-607 Data Sheet

Recombinant Human BMP-4 Protein