Description

Activin A is a dimeric glycoprotein belonging to the transforming growth factor-beta (TGF-β) superfamily, composed of two inhibin β subunits. It can be expressed by various cells in the body, such as endothelial cells, macrophages, and dendritic cells. As a pleiotropic factor, Activin A is widely expressed in tissues and participates in regulating the functions of multiple biological systems, including embryogenesis, cell growth and differentiation, as well as immune and inflammatory responses.

Specifications

Product Details
Cat. No.	Y00101N
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Determined by its ability to inhibit the proliferation of mouse MPC-11 cells. The expected ED50 is ≤ 2.0 ng/ml, corresponding to a specific bioactivity of ≥ 5 x 105 units/mg.
Accssion	P08476
Alternative Names	Activin beta-A chain; Erythroid differentiation protein; EDF
Species	Human
Expression System	CHO
Mol. Weight	12.9 kDa
Expression Region	Gly311-Ser426
Tag Info	Tag free
Formulation	Lyophilized
Buffer	Lyophilized from a 0.22 μm-filtered solution containing 0.085% TFA, 30% ACN, 5% Mannitol, pH2.5.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in 4 mM HCl.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Modulates erythroid and myeloid progenitor differentiation.

Immunology

Regulates immune cell differentiation and inflammatory responses.

Stem Cell Research

Maintains pluripotency of ESCs/iPSCs and guides mesendoderm differentiation.

Cell Biology

Controls cell proliferation, differentiation, and apoptosis via SMAD2/3 signaling.

Data Figures

Figure 1: Recombinant Human Activin A (Cat. No. Y00101N) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y00101N SDS-PAGE.

Determined by its ability to inhibit the proliferation of mouse MPC-11 cells. The expected ED50 is ≤ 2.0 ng/mL, corresponding to a specific bioactivity of ≥ 5 x 105 units/mg.

Figure 2: Determined by its ability to inhibit the proliferation of mouse MPC-11 cells. The expected ED50 is ≤ 2.0 ng/mL, corresponding to a specific bioactivity of ≥ 5 x 105 units/mg.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA