Description

This Recombinant Anti-Human CD3 monoclonal antibody (mAb) is specifically engineered for robust in vitro T cell activation and expansion. By targeting the CD3 subunit on the T cell surface and mimicking TCR signaling, this antibody directly stimulates T cell proliferation without the need for antigen presentation. It is widely utilized as a gold-standard reagent for polyclonal T cell expansion in immunotherapy workflows, including CAR-T cell therapy research, PBMC culturing, and downstream functional T cell assays. When combined with recombinant cytokines such as IL-2, it ensures high-efficiency amplification of target T cell populations.

Specifications

Product Details
Cat. No.	C00404
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤1 EU/mg by the LAL method.
Bioactivity	Measure its binding ability with Jurkat cells using flow cytometry. The ED50 of this effect is less than 10 ng/mL.
Host Species	Mouse
Reactivity	Human
Expression System	CHO
Mol. Weight	150 kDa
Tag Info	Tag free
Formulation	Lyophilized state
Buffer	0.22 μm-filtered solution containing PBS, pH7.4.
Size	500μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Shipping	The product is shipped with blue ice.
Storage & Stability	As supplied (Liquid form): • 12 months at -20°C to -80°C under sterile conditions. • 2-4 weeks at 2°C to 8°C unopened. • Avoid repeated freeze-thaw cycles. Store in aliquots. Note: This product is compatible with lyophilization processes. If lyophilized by user: • 36 months at -20°C to -80°C in lyophilized state. • 6 months at -20°C to -80°C under sterile conditions after reconstitution. • 7-10 days at 2°C to 8°C under sterile conditions after reconstitution.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

T Cell Activation & Proliferation

Used as a primary signal to induce in vitro T cell activation and cross-linking, driving robust T cell proliferation in functional immunological assays.

CAR-T & Cell Therapy Research

An essential component for large-scale T cell expansion and manufacturing protocols in CAR-T cell therapy and adoptive cell transfer (ACT) workflows.

TCR Signaling & Functional Assays

Serves as a stimulating agent to investigate T-cell receptor (TCR) signaling pathways, cytokine release profiles, and immune checkpoint regulations.

Stem Cell Differentiation

Applied in the downstream functional validation and maturation of T cells derived from human pluripotent stem cells (iPSCs/ESCs) or hematopoietic stem cells.

Data Figures

Figure 1: Recombinant Anti-Human CD3 mAb (Cat. No. C00404) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with C00404 SDS-PAGE.

Figure 2: Recombinant Anti-Human CD3 mAb (Cat. No. C00404) binding with Jurkat. The ED50 for this effect is less than 10 ng/mL.

Recombinant Anti-Human CD3 mAb C00404 Biofargo

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA