Description

CD3 monoclonal antibody targets T cells (all T cells express CD3 on their surface, which forms a complex with TCR). CD3 antibodies can be used for T cell activation: by mimicking TCR signaling, they directly activate T cells (without the need for antigen presentation). They are also employed for T cell expansion—in in vitro cultures such as CAR-T cell preparation, CD3 antibodies combined with IL-2 can significantly amplify T cell numbers.

Specifications

Product Details
Cat. No.	C00404
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤1 EU/mg by the LAL method.
Bioactivity	Measure its binding ability with Jurkat cells using flow cytometry. The ED50 of this effect is less than 10 ng/mL.
Species	Mouse
Expression System	CHO
Mol. Weight	150 kDa
Tag Info	Tag free
Formulation	Liquid
Buffer	0.22 μm-filtered solution containing PBS, pH7.4.
Size	500μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Immunology

T cell receptor complex for T cell activation.

Stem Cell Research

Used to activate T cells derived from stem cells.

Cell Biology

Mediates T cell receptor signaling.

Data Figures

Figure 1: Recombinant Anti-Human CD3 mAb (Cat. No. C00404) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with C00404 SDS-PAGE.

Figure 2: Recombinant Anti-Human CD3 mAb (Cat. No. C00404) binding with Jurkat. The ED50 for this effect is less than 10 ng/mL.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA