Descprition

This kit uses a unique lysis buffer system that can quickly release RNA from cultured cell samples for RT-qPCR reactions, thereby eliminating the time-consuming and laborious RNA purification process. The RNA template can be obtained in just 7 minutes. The 5×Direct RT Mix and 2×Direct qPCR Mix-SYBR reagents provided by the kit can quickly and effectively obtain real-time quantitative PCR results.

5×Direct RT Mix and 2×Direct qPCR Mix-SYBR have strong inhibitor tolerance, and the lysate of the samples can be used as the template for RT-qPCR directly. This kit contains the unique RNA high-affinity Foregene reverse transcriptase, and Hot D-Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer.

Product components

Part I	Buffer CL
	Foregene Protease Plus II
	Buffer ST
Part II	DNA Eraser
	5× Direct RT Mix
	2× Direct qPCR Mix-SYBR
	50× ROX Reference Dye
	RNase-Free ddH2O

Features

Simple and effective : with Cell Direct RT technology, RNA samples can be obtained in just 7 minutes.
The sample demand is small, as low as 10 cells can be tested.
High throughput: it can quickly detect RNA in cells cultured in 384, 96, 24, 12, 6-well plates.
DNA Eraser can quickly remove released genomes, greatly reduce the impact on subsequent experimental results.
Optimized RT and qPCR system makes the two-step RT-PCR reverse transcription more efficient and PCR more specific, and more resistant to RT-qPCR reaction inhibitors.

Storage

Part I of this kit should be stored at 4℃; Part II should be stored at -20℃.
Foregene Protease Plus II should be stored at 4℃,do not freeze at -20℃.
Reagent 2×Direct qPCR Mix-SYBR should be stored at -20℃ in the dark; if used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).

Document

QuickEasy™ Cell Direct RT-qPCR Kit-SYBR Green I Instruction Manual-V1.0-EN

QuickEasy™ Cell Direct RT-qPCR Kit–SYBR Green I