Description

Precast-GLgel Hepes Native-PAGE 12% (10-Well) is a ready-to-use, non-denaturing gel designed for analyzing proteins in their native, biologically active state. Constructed with an innovative glass plate design, it effectively reduces non-specific protein adsorption and produces clearer, sharper native protein bands.

This 12% HEPES-based precast gel comes fully prepared with matched Native-PAGE running buffer, allowing direct sample loading without manual gel preparation. The 12% concentration offers balanced resolving capability for medium molecular weight native proteins, and is also suitable for analyzing membrane proteins and native protein complexes, which require gentle separation while maintaining structural integrity.

Specification

Full name	Precast-GLgel Hepes Native-PAGE 12% 10-Well
Package	10 pcs
Components	10 pcs gel, 10 pcs Native-PAGE running buffer
Gel size	8 × 7.4 × 0.15 cm
Plate size	9.8 × 8.4 × 0.41 cm
Wells	10 wells
Buffer system	HEPES buffer system
Maximum sample loading volume	60 µL
Recommended sample loading volume	30 µL

Features

Glass plate construction significantly reduces non-specific protein adsorption, resulting in sharper, clearer native protein bands.
Automatic gel filling technology ensures highly consistent quality and excellent batch-to-batch reproducibility.
12% acrylamide concentration provides optimal resolution for medium molecular weight native proteins.
Suitable for membrane proteins and membrane protein complexes, maintaining structural integrity under non-denaturing HEPES conditions.
Supports separation of native protein complexes, including oligomers and multi-subunit assemblies.
SDS-free gel matrix, ideal for true Native-PAGE studies and downstream functional analysis.
Ready-to-use precast format eliminates manual gel preparation steps and saves significant laboratory time.
Long shelf life with simple storage at 2–8°C.

Applications

Native-PAGE analysis of proteins in non-denatured form
Separation of medium molecular weight native proteins
Electrophoresis of membrane proteins and membrane protein complexes
Analysis of native oligomers and protein complexes
Western blot (WB) after native separation
Experiments requiring a 12% native gel

Storage

2~8°C, do not freeze

Precast-Gel Hepes Native-PAGE 12% 10Well