Description

PLX-5622 is a highly selective brain-penetrant CSF1R inhibitor (IC50=0.016 µM; Ki=5.9 nM) allowing for extended and specific microglial elimination, preceding and during pathology development. Notice: PLX-5622 also works well by intraperitoneal injection (IP), See: Cell Rep. 2022 Aug 16;40(7):111209. Microglia contribute to the postnatal development of cortical somatostatin-positive inhibitory cells and to whisker-evoked cortical activity. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9396528/ Method details Microglia depletion in postnatal pups PLX5622 stock was dissolved in DMSO at a final concentration of 100mg/mL. Pups were injected intraperitoneally every day, from P2 until the day before brain collection, with 0.5μL/g of PLX5622 stock solution diluted in at least an equal volume of DMSO (PLX5622 final concentration is 50μg/g of mouse). PLX-5622 was purchased from Chemgood.

Technical Data

Product Name: PLX-5622

Chemical Formula: C21H19F2N5O

Molecular Weight: 395.41

CAS#: 1303420-67-8

Appearance: Solid powder

Purity: > 99% by HPLC

Solubility: Soluble in DMSO

Storage: Room temperature for months, or -20ºC for 3 years

Targets: CSF-1R

Synonym: PLX-5622; PLX5622; PLX 5622

Chemical Name/IUPAC:3-Pyridinemethanamine, 5-fluoro-N-[6-fluoro-5-[(5-methyl-1H-pyrrolo[2,3-b]pyridin-3-yl)methyl]-2-pyridinyl]-2-methoxy-

SMILES Code: CC1=CN=C2C(C(CC3=C(F)N=C(NCC4=CC(F)=CN=C4OC)C=C3)=CN2)=C1