Synonym(s): 1,4-Piperazinediethanesulfonic acid disodium salt, Piperazine-1,4-bis(2-ethanesulfonic acid) disodium salt, PIPES disodium salt

Description

PIPES-2Na (Disodium Piperazine-1,4-Diethanesulfonate), biochemical grade, is a white crystalline powder with purity ≥ 98%. With a molecular weight of 346.3 and high water solubility, it dissolves to yield clear, colorless solutions suitable for sensitive biochemical applications. As the sodium salt form of PIPES (piperazine-N,N′-bis(2-ethanesulfonic acid)), PIPES-2Na serves as a reliable “Good’s buffer” that helps maintain stable pH under physiological conditions while minimizing interference with metal ions or biomolecules. Its very low UV absorbance makes it ideal for spectrophotometric assays, and its chemical stability supports reproducible results in a wide range of laboratory workflows.

Specifications

Item	Value
Chemical Name	Disodium Piperazine-1,4-Diethanesulphonate
Appearance	White Crystalline Powder
CAS No.	76896-02-7
Molecular Formula	C8H16N2O6S2Na2
Molecular Weight	346.3
Grade	Biochemical Grade
Purity	≥98%
Moisture	≤3.0%
Infrared Spectrum	Qualified
pH (1% Di H2O, 25℃)	9.2–10.0
20% Water Solubility	Colorless, Clear, Transparent
A280 (0.1M in Water)	≤0.050
A260 (0.1M in Water)	≤0.050

Features

High purity & biochemical grade — ensures minimal impurities for consistent experimental results.
Excellent solubility and clarity — dissolves in water to produce clear, colorless solutions.
Stable pH near physiological range — supports cell culture, enzyme assays, and protein work.
Minimal interaction with metal ions — suitable for metal-sensitive biological systems.
Low UV/visible absorbance — compatible with spectrophotometric and fluorescence-based assays.
Chemically stable — maintains integrity under typical laboratory conditions.

Applications

Cell culture media and buffers — maintains stable pH for cell growth and maintenance.
Protein purification and enzyme assays — provides reliable buffering for sensitive reactions.
Electrophoresis and chromatography — effective as running buffer or buffer component.
Microscopy and tissue fixation — preserves cellular structure without interfering with metals.
Protein crystallization and structural biology — supports stable conditions for long-term experiments.

Safety Disclaimer

This product is intended for laboratory research use only. Not for drug, human, or veterinary use. Review the Safety Data Sheet (SDS) provided by BioFargo for detailed handling, storage, hazard, and disposal information. Use appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection when handling. Dispose of waste and residual buffer in accordance with local, state, and federal regulations. Ensure proper ventilation if preparing large volumes or heating solutions.

Documents

MSDS

PIPES-2Na 98% Biochemical Grade