Description

This product is a high-fidelity high-temperature resistant DNA polymerase isolated from the pyrophilus furiosis, with a molecular weight of 90 kDa, with 3'→5' exonuclease activity, no 5'→3' exonuclease activity, and its amplification product is blunt-end without protruding A. The elongation velocity of DNA polymerization was 600 bp/min, and the PCR product was blunt-end. Other high-temperature DNA polymerases, such as Vent, DeepVent, Tli, UlTma, etc., have correction functions, but Pfu has a very low error rate in amplifying DNA among all DNA polymerases that have been discovered so far.

The following catalog numbers have been updated for improved product management:
P310139-0100 → P310139-100U
P310139-0200 → P310139-200U
P310139-0500 → P310139-500U

P310139-5000 → P310139-5KU

Specification

Parameter	Specification
Package	5 KU/100 U/200 U/500 U
Full name	Pfu DNA Polymerase
Composition	Pfu DNA polymerase (5 U/μL), 10X PCR Buffer (20 mM MgSO4).
Reaction Buffer	10X PCR Buffer
Unit Definition	The amount of enzyme required to incorporate 10 nmol of dNTPs into acid-insoluble precipitates at 74°C for 30 min was defined as 1 activity unit (U).
Concentration	5 U/μL
Storage Buffer	20 mM Tris-HCl (pH 8.2); 0.1 mM EDTA; 0.1% Tween 20; 0.1% NP-40; 1 mM DTT; 100 mM KCl; 50% glycerol.
Component	Pfu DNA polymerase (5 U/μL), 10X PCR Buffer (20 mM MgSO4).

Features

The fidelity effect is 8 times higher than that of Taq enzyme.
It is highly thermostable, and the viability of incubation at 95°C for 1 h still maintains more than 90%.
Produces blunt-ended PCR products that bind modified nucleotides (e.g., biotin, digoxigenin, fluorescently labeled nucleotides).

Applications

Conventional PCR, high-fidelity PCR, site-directed mutagenesis, clonal expression.

Storage

-20°C

Pfu DNA Polymerase