Description

PEG 6000 (Polyethylene Glycol 6000) Molecular Biology Grade is a high-purity, non-ionic, hydrophilic polymer designed for molecular biology, biochemical, and life science applications requiring low nuclease contamination and reliable batch consistency. This DNase/RNase-free PEG-6000 is widely used in DNA uptake enhancement, cell fusion, protoplast fusion, macromolecule precipitation, and molecular concentration workflows.

PEG 6000 is readily soluble in water and many organic solvents, while exhibiting excellent biocompatibility, thermal stability, and low chemical reactivity. Due to its hygroscopic and water-binding properties, PEG-6000 is commonly used for concentrating biological samples and improving transformation efficiency in organisms such as yeast and other microbial systems. In molecular biology workflows, PEG 4000 and PEG 6000 are frequently used to promote cell hybridization, facilitate DNA transformation, and assist in biomolecule purification and precipitation procedures.

This product is manufactured to Molecular Biology Grade standards and tested DNase/RNase-free, making it suitable for sensitive nucleic acid handling and research applications.

Specifications

Product Name	PEG 6000
Grade	Molecular Biology Grade
Chemical Formula	HOCH₂CH₂(OCH₂CH₂)_nOH
CAS No.	25322-68-3
EINECS No.	203-473-3
Appearance	White to Off-White Flakes
Average Molecular Weight	6,000 (avg.)
Molecular Weight Range	5400–7800
Water Content	≤0.5%
pH (5% Aqueous Solution)	4.0 – 7.0
Freezing Point	53°C – 59°C
DNase & RNase	Free
Storage	Room Temperature (10–30°C)

Features

Molecular Biology Grade Purity

Manufactured for sensitive molecular biology workflows requiring low nuclease contamination and reliable experimental reproducibility.
DNase/RNase-Free

Tested free of DNase and RNase contamination, suitable for nucleic acid handling, transformation, precipitation, and molecular research applications.
Excellent Water-Binding & Solubility Properties

Highly soluble in water and compatible with multiple aqueous systems, enabling efficient biomolecule concentration and sample processing.
Widely Used for Cell Fusion & DNA Uptake

PEG 6000 is commonly used to promote protoplast fusion, cell hybridization, and DNA uptake in microbial and yeast transformation workflows.
Stable Non-Ionic Hydrophilic Polymer

Exhibits strong biocompatibility, low chemical reactivity, and good thermal stability for diverse laboratory and biochemical applications.
Consistent Batch-to-Batch Performance

Produced under controlled manufacturing conditions to ensure stable molecular weight distribution and reproducible research performance.

Applications

Biofargo PEG 6000 Molecular Biology Grade is widely used in:

Molecular Biology & Nucleic Acid Research

DNA/RNA precipitation, macromolecular crowding studies, nucleic acid concentration, and molecular biology sample preparation workflows.
Cell Fusion & Transformation

Cell hybridization, protoplast fusion, yeast transformation, and enhancement of DNA uptake efficiency in microbial systems.
Protein & Biomolecule Processing

Protein precipitation, biomolecule separation, purification procedures, and therapeutic protein modification research.
Biochemical & Life Science Research

General laboratory research involving hydrophilic polymer systems, sample stabilization, and aqueous formulation studies.
Pharmaceutical & Formulation Development

Used as a suspending agent, thickener, ointment base, excipient, and formulation aid in pharmaceutical and biochemical development workflows.

Documents

SDS

Data Sheet

Disclaimer: For laboratory research use only.

PEG 6000 (Polyethylene Glycol) Molecular Biology Grade, DNase/RNase-Free