Description

This product is designed for the rapid preparation of polyacrylamide gels. It retains Laemmli-like separation characteristics while using a standard Tris-glycine running buffer system.

This kit adopts the pre-mixed formula of the upper gel and the lower gel. After pouring the Lower gel, the upper gel can be directly poured between the glass plates without waiting for the upper gel to polymerize. The upper gel casted by this kit is colored(red, blue or green) for easy sample loading. The odorless catalyst provided by this kit has excellent stability and polymerization effect, and there is no need to add TEMED in the process of casting gels. The gel casted by this kit can be used for denaturing or native PAGE gel electrophoresis.

Features

Ability to cast upper and lower gels at the same time
Colored upper gels(red, blue, green) for easy sample loading
Odorless catalyst instead of TEMED and APS
Improved resolution for small-size (e.g. 10 kDa) proteins

Kit Contents:

Component	Amount
Upper A	80 mL
Upper B	80 mL
Lower A	250 mL
Lower B	250 mL
Catalyst	8 mL

Input Sample Requirements

Similar to the precast gels, the upper gels cast by this kit do not have the ability of conventional stacking.. However, the bands separated by this gel are clearer.
Catalyst for gel polymerization is affected by the temperature. A higher temperature usually accelerates the polymerization, while lower temperature slows the reaction. The volume of Catalyst could be varied according to your purpose.
If the kit is stored at 4°C, please wait until it reaches the room temperature. This could prevent the air bubble production during gels casting.
This product is for research use only.

Storage and Handling

Catalyst: Store at -30 ~ -15°C and transport at ≤0°C. It can be stored at 2 ~ 8°C at least 3 months.
Other components: Store at 2 ~ 8°C. Adjust the shipping method according to the destination.
The kit is stable for at least one year from date of receipt.

Documents

Click here for more guides and user manual.

One step Color PAGE Gel Rapid Preparation Kit