Description

Ni-NTA Sefinose™ Resin 6FF is a high-performance affinity chromatography medium designed for the purification of recombinant proteins with polyhistidine tags. The resin consists of a highly cross-linked 6% agarose matrix covalently coupled to a tridentate chelating agent (Nitrilotriacetic acid, or NTA).By binding $Ni^{2+}$ ions via three coordination sites, this resin provides a stable and high-affinity environment for capturing His-tagged proteins. It is exceptionally versatile, allowing for purification under both native and denaturing conditions from various expression systems, including bacteria (E. coli), yeast, insect, and mammalian cells.Whether you are using gravity flow or automated FPLC systems, Ni-NTA Sefinose™ 6FF delivers high purity with flexible elution options, including imidazole, low pH buffers, or histidine solutions.

Specifications

Item	Value
Matrix	6% Cross-linked Agarose
Chelating Ligand	Tridentate Nitrilotriacetic Acid (NTA)
Binding Capacity	Approx. 10 - 40 mg His-tagged Protein / 1 mL Resin
Average Bead Size	90 μm
pH Stability (Short-term 2h)	pH 2 - 14
Chemical Compatibility	Stable in Common Buffers, Denaturants, and Detergents
Avoid in Buffers	Chelating Agents (EDTA, EGTA), Citrate, Histidine
Storage Solution	20% Ethanol
Storage Temperature	4°C to 8°C (Do Not Freeze)

Features

Superior Selectivity: The tridentate NTA ligand ensures strong Ni^{2+} binding and high specificity for histidine tags, minimizing non-specific protein adsorption.
High-Flow 6FF Matrix: The 90 μm average bead size and 6% cross-linked agarose structure support excellent flow rates, ideal for scaling up from lab to production.
Flexible Binding Capacity: Optimized to bind 10 - 40 mg of His-tagged protein per 1 mL of settled resin.
Robust Chemical Stability: Stable in all commonly used buffers, including various denaturants (Urea, Guanidine-HCl) and detergents (Triton X-100, Tween-20).
Wide pH Range: Exceptional pH stability (Short-term pH 2-14) allows for aggressive Cleaning-in-Place (CIP) protocols.
Multiple Elution Methods: Compatible with Imidazole-based elution, pH shift (low pH), or competitive elution with Histidine.

Applications

Routine His-tag Purification: One-step isolation of recombinant proteins with high recovery.
Inclusion Body Recovery: Efficient purification under denaturing conditions (e.g., 8M Urea or 6M Guanidine-HCl).
FPLC & Gravity Flow: Suitable for pre-packed columns, batch purification, or automated chromatography systems (e.g., ÄKTA).
Versatile Expression Systems: Proven performance with lysates from bacteria, yeast, insect, and mammalian cultures.

Storage

Storage Temperature: Store at 4-8°C; do not freeze.
Storage Buffer: The resin is preserved in 20% ethanol to maintain activity.

Documents

User Manual

Ni-NTA Sefinose(TM) Resin 6FF (Settled Resin)