Description

The Ni-NTA 6FF Pre-Packed Gravity Column (5mL) is a high-performance resin designed for the purification of His-tagged recombinant proteins. This resin is based on a 6% cross-linked agarose matrix, covalently coupled to Nitrilotriacetic acid (NTA), which forms a tridentate chelating agent that binds Ni²+ ions through three coordination sites. This ensures high-affinity binding and efficient purification of His-tagged proteins from various expression systems, including Escherichia coli, yeast, insect cells, and mammalian cells.

The column supports both native and denaturing conditions during purification and provides versatility in elution strategies, whether with a low pH buffer, imidazole, or even histidine solution. With an average bead size of 90 μm and a protein binding capacity of 10-40 mg of His-tagged protein per 1 mL resin, the Ni-NTA 6FF column ensures reliable and reproducible results with minimal non-specific binding.

This resin is compatible with all commonly used buffers, detergents, and denaturants, making it a reliable choice for researchers. The column’s stability across a wide pH range (2-14) makes it adaptable to various experimental conditions. Additionally, the resin can be regenerated and reused up to 5-10 times, making it a cost-effective solution for ongoing purification needs.

Specification

Item	Value
Composition	5 mL (1/5/10 columns)
Substrate	6% Agarose
Average Particle Size	0
Particle Size	45 μm - 165 μm
Protein Binding Ability	Approximately 10-40 mg Histidine Labeled Protein / 1 mL Resin
Maximum Flow Rate	150 cm/h
Pressurization	0.3 MPa, 3 bar
Storage Buffer	20% Ethanol
Component	5 mL (1/5/10 columns)
Storage	4-8°C, do not freeze. Store in 20% ethanol solution

Features

High-Affinity Purification: The Ni-NTA resin binds Ni²+ ions via three coordination sites, offering a high-affinity interaction for His-tagged proteins.
Broad Compatibility: Suitable for purification from multiple expression systems (E. coli, yeast, insect, and mammalian cells), under both native and denaturing conditions.
Optimal Purity: The resin offers minimal non-specific adsorption, enabling high-purity isolation of His-tagged proteins.
Wide pH Stability: Remains stable in pH 2-14, with short-term use for up to 2 hours at extreme pH values.
Protein Binding Capacity: 10-40 mg of His-tagged protein per 1 mL resin, depending on conditions and protein characteristics.
Regenerative and Reusable: The column can be reused 5-10 times, providing excellent cost efficiency.
Chemical Compatibility: Stable in most commonly used buffers, detergents, and denaturants; however, avoid chelating agents like EDTA, EGTA, citrate, and histidine.

Applications

His-tagged protein purification: Ideal for purifying recombinant proteins from E. coli, yeast, insect, and mammalian cells.
Protein refolding: Suitable for refolding proteins from denatured states during purification.
Affinitive chromatography: Uses metal affinity chromatography for high-purity protein isolation in biotech, pharmaceutical, and biochemistry labs.

Storage

Storage Temperature: Store at 4-8°C; do not freeze.
Storage Buffer: The resin is preserved in 20% ethanol to maintain activity.

Documents

User Manual

Ni-NTA 6FF(Pre-Packed Gravity Column),5mL