Description

The Ni-NTA Sefinose Resin is an advanced agarose resin (6% cross-linked) covalently coupled to a tridentate nitrilotriacetic acid (NTA) chelating agent, which enables high-affinity binding of Ni²+ ions. This resin is designed for the purification of His-tagged recombinant proteins from various expression systems, including bacteria, yeast, insect cells, and mammalian cells. His-tagged proteins can be efficiently purified under both native or denaturing conditions, with the resin offering high selectivity and reproducibility.

Purification is achieved by eluting bound proteins with a low pH buffer, imidazole, or even histidine solution, making it ideal for researchers seeking a high-purity protein purification system. The resin supports a wide range of experimental conditions and can be used in small-scale laboratory or large-scale industrial applications.

Specification

Item	Value
Composition	1 Kit (1 mL × 10 Columns + 500 mL Elution Buffer + 500 mL Binding/Wash Buffer)
Substrate	Highly Cross-linked 6% Agarose
Average Particle Size	90 μm (45-165 μm)
Particle Size	0
Protein Binding Ability	Approximately 10-40 mg Histidine Labeled Protein / 1 mL Resin
Maximum Flow Rate	150 cm/h
Pressurization	0.3 MPa, 3 bar
Storage Buffer	20% Ethanol
Component	1 Kit (1 mL × 10 Columns + 500 mL Elution Buffer + 500 mL Binding/Wash Buffer)
Storage Conditions	Store in 20% Ethanol at 4-8°C. Do Not Freeze

Features

High Binding Capacity: The resin has a protein binding capacity of approximately 10-40 mg His-tagged protein per 1mL resin, ensuring effective protein capture and purification.
Excellent Selectivity: NTA ligands on the resin significantly reduce non-specific adsorption, resulting in high-purity proteins post-purification.
Stable Across a Broad Range of Conditions: The resin is stable in commonly used buffers, denaturants, and detergents, offering versatility across various experimental conditions.
Wide pH Stability: Stable in the pH range of 2-14 for up to 2 hours (Microsphere test), enabling use in diverse research and industrial applications.
Ideal for Large-Scale Purification: With an average bead size of 90 μm and a high flow rate, this resin is perfect for high-throughput and large-scale protein purification.
Economical and Reusable: The resin can be regenerated and reused multiple times, making it a cost-effective solution for repeated protein purification runs.

Application

Purification of His-Tagged Proteins: Ideal for purifying His-tagged recombinant proteins from E. coli, yeast, insect cells, and mammalian systems.
Protein Refolding: Perfect for the refolding of denatured proteins directly during the purification process, making it suitable for inclusion body protein recovery.
High-Throughput Applications: With its high flow rate and large-scale compatibility, this resin is well-suited for high-throughput protein production in both research and industrial settings.
Optimized Elution Options: Elution of bound proteins can be performed with low pH buffer, imidazole, or histidine solution, providing flexibility in your purification protocol.

Storage

Store in 20% ethanol at 4-8°C. Do not freeze.

Documents

User Manual

Ni-NTA 6FF Sefinose(TM) Resin Kit