Description

MycoSHENTEK™ Mycoplasma DNA Detection Kit (2G) is used, together with MycoSHENTEK™ Mycoplasma DNA Extraction Kit (2G), to qualitatively determine whether there is mycoplasma, spiroplasma, or acholeplasma contamination in master cell bank, working cell bank, virus seed stock, or cell culture-derived products, etc. The integrated system has been fully validated per European Pharmacopoeia Chapter 2.6.7 and Japanese Pharmacopoeia XVII, at a detection limit of 10 CFU/mL.
The detection kit uses real-time PCR technology to detect more than 200 species of Mycoplasma, Spiroplasma and Acholeplasma with high specificity. It has been validated in a variety of matrices, as well as on non-mycoplasma species, production cells, and engineered bacteria and fungi.

Introduction

Components	Shipping Condition	Unit Size	Product Line	Detection Method
MyqPCR reaction buffer (2G)	-20℃, protect from light	50 Reactions	MycoSHENTEK	Probe-based qPCR
MyPrimer & Probe MIX (2G)	-20℃, protect from light
Internal control (IC) (2G)*	-20℃
Positive control (PC) (2G)*
DNA dilution buffer

Specification

Inclusivity	More than 180 species of Mycoplasma, Spiroplasma and Acholeplasma
Detection Limit	10 CFU/mL or 1 genome copy/μL (Positive cut-off: 95%)
Specificity	No matrix-induced effect across different sample matrices and no cross-reactivity to nearly 30 types of cell lines
Robustness	Freeze-thaw stability over 5 cycles
	Instrument suitability not limited to Thermo, Biorad, Roche, HZSKBio qPCR equipments
	Consistent LOC in different matrices

Documents

User Guide

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Mycoplasma DNA Detection Kit (2G) MycoSHENTEK™