Description

MycoSHENTEK™ Mycobacteria DNA Detection Kit is used, together with MycoSHENTEK™ Mycobacteria DNA Extraction Kit, to qualitatively determine whether there is Mycobacteria contamination in cells, cell culture-derived biologicals, etc., using real-time qPCR techonology. The integrated system has been fully validated at a detection limit of 10-100 CFU/mL to detect more than 100 different Mycobacteria species. The system contains dUTP, and if used with the UNG enzyme to prevent contamination and eliminate carry-over contamination Internal Control (IC) can be added to a PCR amplification reaction mixture to evaluate whether a test sample inhibits the amplification reaction and may result in false negative results. It can also be added to the sample before extraction to evaluate the extraction efficiency. For extraction information, please refer to MycoSHENTEK™ Mycobacteria DNA Extraction Kit User Guide (Product No.: 1503601).

Introduction

Components	Shipping Condition	Unit Size	Product Line	Detection Method
MB qPCR Reaction Buffer	-20℃, protect from light	50 Reactions	MycoSHENTEK	Probe-based qPCR
MB Primer & Probe MIX	-20℃, protect from light
MB Internal Control (IC)	-20℃
MB Positive Control (PC)
DNA Dilution Buffer (DDB)

Specification

Inclusivity	More than 100 different Mycobacteria species
Detection Limit	10 CFU/test or 20 copies/test (Positive cut-off: 95%)
Specificity	No matrix-induced effect across different sample matrices and no cross-reactivity to different cell lines, bacteria or fungi
Robustness	Freeze-thaw stability over 5 cycles
	Instrument suitability not limited to Thermo, Biorad, Roche, Jena, HZSKBio qPCR equipments
	Consistent performance to the slight variations of reaction system and amplification procedure

Documents

User Guide

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Mycobacteria DNA Detection Kit MycoSHENTEK™