Synonym(s): 3-(N-Morpholino)propanesulfonic acid, 4-Morpholinepropanesulfonic acid, MOPS, Free Acid, ULTROL® Grade, 3-Morpholinopropane sulfonic acid

Description

In biological experiments, it is an important pH stabilizing reagent. Typically, a suitable weak acid is mixed with its conjugate base to obtain the appropriate pH value. Most biological reactions occur under neutral conditions, generally with a pH between 6 and 8, so the effective buffering range of the buffer is also required to be between 6 and 8. In addition, it is required that the acidic and basic forms of the buffer do not chelate with certain metal ions.

Specifications

Item	Value
Chemical Name	3-Morpholinopropanesulfonic Acid MOPS
Appearance	Pure White Powder
CAS No.	1132-61-2
Molecular Formula	C₇H₁₅NO₄S
Molecular Weight	209.3
Grade	High Purity Grade
Purity	≥99.0%
Heavy Metals (Total)	≤5ppm
Heavy Metals (Iron)	≤5ppm
pH	2.5-4.5
Loss on Drying (105°C, 3 hours)	≤0.18%
Solubility (10% in water)	Passed The Test
UV Light Absorption	≤0.02abs Unit
Storage / Handling	Store at Room Temperature.

Features

High-purity biochemical grade (≥99%)

Ensures reliable performance for molecular biology, biochemistry, cell culture, and analytical applications requiring strict quality control.
Optimal buffering near physiological pH

With a pKa of approximately 7.2, MOPS provides excellent buffering capacity in the 6.5–7.9 range, ideal for biological reactions conducted under near-neutral conditions.
Low UV absorbance for sensitive assays

Exhibits minimal absorbance in the UV range, ensuring compatibility with spectrophotometric, nucleic acid, and protein quantification assays without introducing interference.
Minimal metal-ion binding

As a Good’s buffer, MOPS shows negligible chelation with divalent metal ions, maintaining the integrity of metal-dependent enzymes and minimizing experimental artifacts.
High stability and excellent solubility

Available as a stable, pure white powder that dissolves easily in water to produce clear, colorless solutions. Suitable for long-term storage at room temperature.

Applications

Electrophoresis buffers

Widely used in RNA and DNA gel electrophoresis (such as MOPS running buffer for formaldehyde agarose gels) to ensure stable pH and high-resolution band separation.
Cell culture media buffering

Suitable for maintaining physiological pH in mammalian, bacterial, or yeast culture media, particularly in CO₂-independent or serum-reduced systems.
Protein purification workflows

Used in chromatography buffers (ion-exchange, SEC, affinity purification) due to its stable buffering action and non-chelating properties that preserve protein structure and activity.
Enzyme reactions and biochemical assays

Ideal for enzymatic kinetics, activity measurements, and biochemical reactions requiring precise pH control near neutrality.
Sample preparation and extraction

Utilized in buffers for cell lysis, nucleic acid extraction, and protein isolation where consistent pH and low interference are essential for downstream analysis.
Metal-ion-sensitive experiments

Recommended for assays involving metal-dependent enzymes or pathways where avoiding metal ion chelation is critical for accurate results.

Safety Disclaimer

This product is intended for laboratory research use only. Not for drug, human, or veterinary use. Review the Safety Data Sheet (SDS) provided by BioFargo for detailed handling, storage, hazard, and disposal information. Use appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection when handling. Dispose of waste and residual buffer in accordance with local, state, and federal regulations. Ensure proper ventilation if preparing large volumes or heating solutions.

Documents

COA

SDS

MOPS Free Acid 99% High Purity Grade