Description

Beef extract and peptone acts as source of carbon, nitrogen, minerals, vitamins and other essential growth nutrients. Mannitol is the fermentable carbohydrate. Phenol red is a pH indicator and change the color from red to yellow if mannitol is fermented and acid products are produced. Typical pathogenic staphylococci (coagulase-positive staphylococci) ferment mannitolandform yellow colonies with yellow zones around the colonies. Typical non-pathogenic staphylococci do not ferment mannitol andform red colonies. Sodium Chloride, in high concentration, inhibits most bacteria other than staphylococci.

Cultural characteristics after 18-48 hours at 35-37°C.

Components (for making 4.55L medium)

Part I:

Protease pepton 45.5g
Sodium Chloride 341.25g
D-Mannitol 45.5g
Beef Extract 4.55g
Phenol Red 0.115g

Part II:

Agar 68.25g

Usage

Must dissolve all contents of Part I in distilled water. Adjust pH=7.4-7.5, make final to 4.55L. Add Part II (Agar).

Sterilize by autoclaving at 121°C for 15 minutes. Pour plates.

Storage

Store dehydrated powder, in a dry place, in tightly-sealed containers at 2-25°C.

DG:No

Storage:18 to 25℃

Sterile:No

Documents

Product Info

Mannitol Agar,1kg