Description

The kit adopts magnetic beads with unique separation function and a unique buffer system to separate and purify high-quality total RNA from various tissues, cells and blood. The product can be perfectly matched with Kingfisher Flex 96 and TGuide S32 automated nucleic acid extractors. Magnetic beads are adsorbed, transferred and released by special magnetic rods, thus realizing the transfer of magnetic beads and nucleic acids and improving the automation degree. The whole experimental process is safe and convenient, with high purity extracted total RNA, free from contamination of genomes, proteins and other impurities. If high-throughput automated extraction is required, TIANGEN Company can provide an integration plan.

Features

The kit can meet the requirements of manual extraction as well as batch extraction on various high-throughput platforms.
The products obtained by the kit meet the requirements of downstream detection experiments and NGS analysis.

Applications

RNA purified by the kit is suitable for RT-PCR, Real Time RT-PCR, chip analysis, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, molecular cloning and other downstream experiments.

Kit Contents

Contents	50 preps
Buffer RL	30 mL
Buffer RD	48 mL
Buffer RW	2 × 12 mL
RNase-Free ddH₂O	15 mL
MagAttract Suspension W	2 × 1 mL

Required Reagents

β-mercaptoethanol, ethanol.

Storage

The kit can be stored for 15 months under dry conditions at room temperature (15-30°C) and stored at 2-8℃ for a longer time.

Important Notes

Before operation, add β-mercaptoethanol to Buffer RL to a final concentration of 1%, for example, add 10 μl of β-mercaptoethanol to 1 ml of Buffer RL. This buffer is best prepared right before use. The prepared Buffer RL can be stored at 2-8°C for 1 month. The Buffer RL may form precipitates during storage, and if any precipitates appear, please heat and dissolve before use.
Add 96-100% ethanol to Buffer RD and Buffer RW before the first use. Please refer to the label on the bottle for the added volume.

Experimental Example

Optional Reagents and Tools

DNase I (Cat.no. GRT411); Magnetic Stand (Cat.no. OSE-MF-01); 10×Red cell Lysis Buffer H; RNase-Free Columns CS set.

Self-provided Reagents and Instruments

β-mercaptoethanol, isopropanol, 96-100% ethanol, homogenization equipment (mortar, electric homogenizer, etc.), magnetic stand.

Documents

GDP761-Magnetic Tissue Cell Blood Total RNA Kit-240514

Magnetic Tissue/Cell/Blood Total RNA Kit