Description

Laboratories isolating human NK cells from whole blood need to remove non-NK cells while keeping the target NK cells unlabeled for downstream experiments. The K005 Human NK Cell Purification Cocktail provides a negative-selection workflow for human whole blood. Its antibodies recognize specific surface markers on non-target cells and crosslink these cells to multiple red blood cells, forming dense immunorosettes.

During density gradient centrifugation, the immunorosettes sediment together with free red blood cells. The target human NK cells remain unlabeled and become highly enriched at the interface between the plasma and the density gradient medium, where they can be collected for subsequent laboratory use.

This human NK cell purification cocktail is supplied as one kit in a 3 mL inner container measuring 16 mm × 35 mm. The product weight is 5 g. Before use, reconstitute the cocktail with 2 mL of sterile water. The recommended working amount is 40 µL of cocktail per 1 mL of human whole blood.

Important: Density gradient separation medium is not included with this product and must be supplied separately by the user.

Specification

Product Name	Human NK Cell Purification Cocktail
Catalog Number	K005
Specification	1 Kit
Quantity	1
Inner Packaging Dimensions	16 mm × 35 mm
Inner Container Size	3 mL
Product Weight	5 g
Sample Type	Human whole blood
Selection Method	Negative selection using immunorosette formation and density gradient centrifugation
Reconstitution	Reconstitute with 2 mL of sterile water
Recommended Working Amount	40 µL per 1 mL of blood sample
Recommended Incubation	20 minutes at room temperature
Recommended Sample Condition	Whole blood collected within 24 hours and stored at 15–25°C
Storage	−20°C
Thawing Before Use	Transfer to 4°C in advance to thaw
Density Gradient Separation Medium	Not included; must be supplied separately
Tube Compatibility	Use standard centrifuge tubes; not compatible with lymphocyte separation tubes
Intended Use	For research use and further manufacturing only; not for direct use in humans

The 3 mL specification refers to the inner container size. The product is reconstituted with 2 mL of sterile water before use.

Features

Human NK cell purification from human whole blood.
Negative selection based on antibody-mediated immunorosette formation.
Non-target cells are crosslinked to multiple red blood cells.
Immunorosettes and free red blood cells sediment during density gradient centrifugation.
Target NK cells remain unlabeled and are collected from the interface between the plasma and density gradient medium.
Recommended working amount of 40 µL per 1 mL of blood sample.
Supports workflows with or without the preparation of autologous inactivated plasma.

Application

The K005 Human NK Cell Purification Cocktail is intended for the negative selection and enrichment of human NK cells from human whole blood. Following purification, the collected NK cells are suitable for the downstream applications specified below:

Flow cytometry
Cell culture
DNA extraction
RNA extraction

This product is for research use and further manufacturing only and is not intended for direct use in humans.

FAQ

What is the K005 Human NK Cell Purification Cocktail used for?

K005 is used to enrich human NK cells from human whole blood by negative selection. The purified cells can be used for flow cytometry, cell culture, or DNA/RNA extraction.

How does the Human NK Cell Purification Cocktail work?

The antibodies in the cocktail recognize specific surface markers on non-NK cells and crosslink these cells to multiple red blood cells. The resulting dense immunorosettes sediment with free red blood cells during density gradient centrifugation, while the target NK cells remain unlabeled at the interface between the plasma and density gradient medium.

Can K005 be used for NK cell isolation directly from human whole blood?

Yes. The documented sample type is human whole blood. For optimal performance, use whole blood collected within 24 hours and stored at room temperature between 15°C and 25°C.

How should K005 be reconstituted and used?

Reconstitute the Human NK Cell Purification Cocktail with 2 mL of sterile water. The recommended working amount is 40 µL of cocktail per 1 mL of blood sample, followed by incubation at room temperature for 20 minutes.

Is density gradient separation medium included with K005?

No. Density gradient separation medium is not included with the product and must be supplied separately by the user.

What other materials are required for human NK cell purification?

The documented workflow requires human serum albumin at 20% concentration, Ficoll density gradient medium, DPBS, standard centrifuge tubes, pipettes, and other standard laboratory consumables. These materials are not identified as components of the K005 kit.

Can lymphocyte separation tubes be used with this product?

No. K005 is not compatible with lymphocyte separation tubes. Standard centrifuge tubes must be used for blood separation.

How should the Human NK Cell Purification Cocktail be stored?

Unused product should be stored at −20°C. Before use, transfer the product to 4°C in advance to thaw.

What centrifugation conditions are used for density gradient separation?

After layering the diluted sample onto the density gradient medium, centrifuge at 1200 × g for 20 minutes using acceleration setting 1 and deceleration setting 0. Harvest the buffy coat layer and wash at 300 × g for 10 minutes using acceleration setting 8 and deceleration setting 6. Repeat the wash step once.

What blood volumes can be processed?

The workflow without preparation of autologous inactivated plasma specifies up to 15 mL of fresh whole blood per tube. The workflow that includes preparation of autologous inactivated plasma specifies up to 50 mL of fresh whole blood per tube.

Human NK Cell Purification Cocktail – K005