Product Description

The human IFN-γ ELISA kit is used for the in vitro quantitative detection of human IFN-γ in human serum, plasma, buffer or cell culture supernates . It can detect both natural and recombinant human IFN-γ.This kit is a pre-coated plate, which is completed by "sandwich one step". The incubation time of the whole process does not exceed 3 hours, and the total washing time is 6 times which greatly reduces the experimental time.

Product Details

Detection range : 12.5-400 pg/mL

Sensitivity: 5 pg/mL

Precision : Intra-Assay CV: ≤10%; Inter-Assay CV: ≤15%

Stored : 2 - 8 °C

Verified Reactivity：Human

Reagents Provided and Reconstitution

Reagents	Quantity	Reconstitution
Cytokine standard	2 vials	Lyophilized, reconstitute with the volume of reagent indicated on the vial
Biotinylated antibody	2 vials	Dilute 50 times in Diluent buffer（1×）
Streptavidin-HRP	2 vials	Dilute 100 times in Diluent buffer（1×）
Diluent buffer（1×）	3 vials	Ready-to-use
Washing buffer（50×）	1 vial	Dilute 50 times in distilled Water
TMB	1 vial	Ready-to-use
Stop solution	1 vial	Ready-to-use
Precoated ELISA plates	8×12	Ready-to-use
Plastic cover	4 sheets	Ready-to-use

Material Required but not Provided

96-well ELISA plate (microplate spectrophotometer).
Pipettes and pipette tips: 10 μL, 50 μL, 100 μL, 200 μL and 1000 μL.
Distilled water, fresh absorbent paper.
Vortex mixer and magnetic stirrer

Scientific Data

Assay Procedure Summary

Add 100 μL of sample, standard or control per well
Add 50 μL of diluted Biotinylated Detection antibody to all wells
Incubate 2 hours at room temperature
Wash three times
Add 100 μL of streptavidin-HRP to all wells
Incubate 20 minutes at room temperature
Wash three times Add 100 μL of ready-to-use TMB Avoid direct exposure to light. Let the color develop for 10-30 minutes.
Add 100 μL Stop solution to all wells
Read at 450 nm within 15 minutes

Document

Technical Data Sheet

Human IFN-γ Precoated ELISA kit-96T-BioSci™