Background

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins, which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.

Features

Specification: 96 Test
Sensitivity: 0.64 pg/ml (50 μl); 2.62 pg/ml (10 μl)
Standard Curve Range: 3.13–200 pg/ml
Standard Curve Gradient: 7 Points
Number of Incubations: 2
Sample Volume: 50 μl / 10 μl
Assay Type: Sandwich ELISA
Operation Duration: 120 min

Typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0190	0.0200	0.0195
3.13	0.0300	0.0310	0.0305	0.0110
6.25	0.0520	0.0500	0.0510	0.0315
12.50	0.1150	0.1140	0.1145	0.0950
25.00	0.2920	0.2800	0.2860	0.2665
50.00	0.7590	0.7000	0.7295	0.7100
100.00	1.8220	1.7240	0.7295	1.7535
200.00	3.2790	1.7240	3.1850	3.1655

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average(pg/ml)	4.6	18.4	58.9	4.2	19.5	59.7
Standard Deviation	4.6	0.59	4.1	0.21	0.72	3.8
Coefficient of Variation(%)	2.6	3.2	6.9	5.0	3.7	6.4

Intra-assay Precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (precision between assays): Three samples of known concentration were tested six times on different plates to assess inter-assay precision.

Spike Recovery

Spike recovery was evaluated by spiking three levels of HIV-1 p24 into serum samples. Unspiked serum was used as a blank in this experiment.

The recovery ranged from 94% to 105%, with an overall mean recovery of 98%.

Storage

Store at 4 °C. Please refer to the protocols for detailed instructions.

Research Use Only

For research purposes only. Not intended for use in diagnostic procedures.

Documents

HIV-1 p24 ELISA Kit