Description

FREY agar is a nutritionally enriched, semi-selective solid medium designed for the cultivation and detection of avian mycoplasmas. The formulation is supplemented with nicotinamide adenine dinucleotide (NAD⁺), a critical coenzyme required for the growth of NAD-dependent species such as Mycoplasma synoviae.

The presence of phenol red as a pH indicator enables visual monitoring of metabolic activity, with a color shift from red to yellow (acidic) or purple (alkaline) indicating the proliferation of mycoplasmal organisms. To selectively inhibit contaminating bacterial flora while preserving mycoplasma viability, penicillin G is incorporated into the medium.

Furthermore, the addition of exogenous lipid supplements from yolk, excluding antibiotics—including cholesterol and long-chain fatty acids—enhances the growth performance of lipid-requiring strains, particularly Mycoplasma pneumoniae ATCC 23714, by supporting membrane biosynthesis and promoting optimal colony formation.

Specifications

Agency	EP 2.6.7 / USP / JP
Quality Level	ISO 9001
Sterility	Sterile
-	Aseptically Filled
Form	Plate
Feature	Ready-to-use; ICR Settle plates
packaging	Pack of 10 plates; Box of 10 packs
Plate Diam	60 mm
pH	7.8 (in H2O)
Application(s)	Industrial qc
Storage Temp	2-8℃ for 3 months
Suitability	Mycoplasma

Application

FREY agar is recommended for the detection of avian mycoplasmas in pharmaceutical products, particularly in bulk vaccines, cell banks, and virus cultures.

Features

Ready-to-use convenience — no additional preparation steps are required.
Broad pharmaceutical applications — suitable for biopharmaceuticals, vaccines, cell cultures, and virus cultures.
Storage stability — can be stored at 2–8 °C for up to 3 months.
Rapid detection — a colorimetric chart provides a quick and convenient visual indication of early-stage mycoplasma proliferation.
Optimized growth performance — the buffered system supports higher colony counts while reducing the risk of mycoplasma cell death.
Enhanced visualization — a chromogenic reagent selectively stains mycoplasma, improving clarity and specificity of detection under microscopy.
Improved NAD⁺ stability — to prevent growth inhibition caused by NAD⁺ degradation at later stages, alternative NAD⁺ analogs are co-supplemented in the culture system.

Documents

M-FREY-X SDS

Frey Agar (M-FREY-X)