Question 1

How does X3 compare with Qubit fluorometers?

Accepted Answer

X1 is a single-sample instrument, while X3 provides eight sample positions. Qubit 4 is also a single-sample instrument and Qubit Flex measures up to eight samples. Optical filters, consumables, software, assay menus and validation differ; matching sample capacity does not establish equivalent analytical performance.

Question 2

Which reagents can I use?

Accepted Answer

The technical guide lists Biofargo BF-T1002, BF-T1004, BF-T1005, BF-T1006, BF-T1007 and BF-T1008-V2 and selected Qubit reagents. Use the matching assay program, kit standards and instrument-specified tubes. Compatibility is based on the supplier guide, not Thermo Fisher endorsement.

Question 3

Which tubes does X3 require?

Accepted Answer

Instrument-specified clear 0.2 mL PCR single tubes or eight-tube strips; flat, straight caps required. Use the tube geometry specified in the technical guide; the 0.5 mL tubes used by X1 cannot be substituted for X3 tubes.

Question 4

Is the read time the total assay time?

Accepted Answer

No. The specification is ≤5 seconds per sample. Preparation, calibration and incubation add time. It does not establish that eight samples are measured together in five seconds.

Question 5

How do I calibrate the assay?

Accepted Answer

Select the appropriate program and read the two standards supplied with the chosen kit. For the listed nucleic acid assays, prepare each standard with 10 µL of standard and 190 µL of working solution. Enter the correct sample input volume before reading unknowns. Do not mix standards or calibration curves from different kits.

Question 6

Can I export results?

Accepted Answer

Yes. Insert a USB flash drive and use the data-query/export function described in the technical guide.

Question 7

What is included with the instrument?

Accepted Answer

The technical packing list includes the fluorometer, power cord, USB drive with user guide and a packing list. Assay reagents and assay tubes are separate purchases.

Question 8

Is the instrument intended for diagnostic use?

Accepted Answer

No. For research use only. Not for diagnostic or therapeutic procedures.

Parameter	Specification
Biofargo catalog number	BF-M2003
Sample positions	8
Sample input	1–20 µL; depends on assay
Assay volume	200 µL for the listed nucleic acid protocols; follow the selected assay instructions
Compatible tubes	Instrument-specified clear 0.2 mL PCR single tubes or eight-tube strips; flat, straight caps required
Read time	≤5 seconds per sample; not a stated eight-sample batch time
Optical channels	Blue (460–480 nm excitation / 500–535 nm emission) and red (630–650 nm excitation / 670–710 nm emission)
Detector	SiPMT (silicon photomultiplier)
Assay programs	dsDNA HS, dsDNA BR, RNA HS, RNA BR, RNA XR, ssDNA, microRNA and Protein BR
Calibration	Two-point assay calibration using matched standards
Display	5-inch touchscreen
Data export	USB flash drive
Instrument power	DC 5 V, 2 A; maximum 10 W
Operating conditions	Indoor use; 15–30 °C; relative humidity ≤75%; altitude up to 2,000 m
Transport/storage temperature	−20 to 60 °C
Weight	0.5 kg
Intended use	Research use only
Data storage	8 GB; up to 20,000 records

Assay Kit	Analyte	Assay Range
Biofargo dsDNA HS Assay Kit	Double-stranded DNA	0.1–120 ng
Biofargo 1X dsDNA HS Assay Kit	Double-stranded DNA (ready-to-use 1X)	0.2–100 ng
Biofargo dsDNA BR Assay Kit	Double-stranded DNA	2–1,000 ng
Biofargo RNA HS Assay Kit	RNA	5–100 ng
Biofargo RNA BR Assay Kit	RNA	20–1,000 ng
Biofargo ssDNA Assay Kit-V2	Single-stranded DNA	1–200 ng
Biofargo Fluorometric Protein Quantification Kit	Protein	0.25–5 µg

Description

Specifications

Features

Applications

Frequently Asked Questions (FAQs)

Compatible Assay Kits

Documents

Biofargo Fluorometer X3