Description

This product includes an enhanced luminol substrate and a stable peroxide system to detect femtogram amounts of antigen and is suitable for chemiluminescence by conventional Western Blot. This product contains an enhanced luminol substrate and a stable peroxide system for detecting femtogram amounts of antigen and is suitable for chemiluminescent detection in conventional Western Blot.

Features

Stability:

24-hour working solution stability; 1-year kit stability at room temperature.
High Cost Performance:

Compared with other chemiluminescent substrates of the same grade, the cost performance is quite high.
High Sensitivity:

Quickly detect proteins as low as femtogram amounts. Its performance is significantly better than other high-end brand products of the same level.

Ultra-high sensitivity at real femtogram level

The 1 mg/mL actin-HRP antibody was serially diluted to obtain concentrations of 1000, 500, 250, 125, and 62.5 fg/μL. 1μL of each dilution was spotted onto an NC membrane. After spotting, the membrane was developed with YamayBio VQ4726 for 5 minutes.

ECL Western Blotting Ultimate Sensitivity Substrate -Femto-Yamaybio

YamayBio VQ4726 offers higher femtogram-level sensitivity than Thermo 37071.

Lysate: A431 whole cell lysates MW: GAPDH-36kD Primary antibody: Anti-GAPDH, dilution at 1:5000 Secondary antibody: Goat anti-Mouse IgG(H+L),HRP conjugated at 1:5000 dilution Exposure duration: 10 seconds.

ECL Western Blotting Ultimate Sensitivity Substrate -Femto-Yamaybio

Specifications

Product Type	Substrate
Detection Method	Chemiluminescence
Form	Liquid
Application	Western Blot
Quantity	100 mL / 500 mL
Sensitivity	Femtogram
Membrane Compatibility	Nitrocellulose, PVDF

Storage

Transported at room temperature, stored at 4°C away from light, shelf life is 12 months.

Documents

VQ4726-100 COA

VQ4726-500 COA

SDS

User Guide

Flyer of Product_ Western Blot Substrate Kit

ECL Western Blotting Ultimate Sensitivity Substrate-Femto-Yamaybio